Energy status and its control on embryogenesis of legumes:: ATP distribution within Vicia faba embryos is developmentally regulated and correlated with photosynthetic capacity
Energy status and its control on embryogenesis of legumes:: ATP distribution within Vicia faba embryos is developmentally regulated and correlated with photosynthetic capacity
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DOI：
10.1046/j.1365-313x.2003.01879.x
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发表时间：
2003-11-01
期刊：
影响因子：
7.2
通讯作者：
Weber, H
中科院分区：
文献类型：
--
作者：
Borisjuk, L;Rolletschek, H;Weber, H
为了分析蚕豆胚胎分化过程中的能量状态，我们使用定量生物发光成像技术直接在冷冻切片中测量ATP浓度。该方法提供了接近体内情况的ATP分布的定量图像。ATP浓度始终最高的轴内。在贮藏前的子叶中，该水平较低，但在进一步的发育过程中，从子叶远轴区开始，向内部迅速增加。绿化模式，叶绿素分布和光合O-2产生的胚内的时间和空间对应的ATP分布，暗示的能量状态的整体增加与绿化过程。ATP模式与胚胎的光合能力有关。ATP的一般分布模式以及稳态水平受发育调节，在黑暗/光照条件下不发生变化。主要的贮藏蛋白豆球蛋白开始积累在远轴区域与高ATP，而淀粉定位在区域与相对较低的ATP水平。这表明了同化物分配到不同的存储产品类的能量状态的作用。最高的生物合成速率发生时，子叶成为完全绿色，并含有高ATP水平，这意味着需要一个光异养状态，以确保高通量。基于这些数据，我们提出了一个模型，胚胎光合作用的作用，以改善胚胎的能量状态。
To analyse the energy status of Vicia faba embryos in relation to differentiation processes, we measured ATP concentrations directly in cryosections using a quantitative bioluminescence-based imaging technique. This method provides a quantitative picture of the ATP distribution close to the in vivo situation. ATP concentrations were always highest within the axis. In pre-storage cotyledons, the level was low, but it increased strongly in the course of further development, starting from the abaxial region of cotyledons and moving towards the interior. Greening pattern, chlorophyll distribution and photosynthetic O-2 production within embryos temporally and spatially corresponded to the ATP distribution, implicating that the overall increase of the energy state is associated to the greening process. ATP patterns were associated to the photosynthetic capacity of the embryo. The general distribution pattern as well as the steady state levels of ATP were developmentally regulated and did not change upon dark/light conditions. The major storage protein legumin started to accumulate in abaxial regions with high ATP, whereas starch localized in regions with relatively lower ATP levels. This suggests a role of the energy state in the partitioning of assimilates into the different storage-product classes. Highest biosynthetic rates occurred when cotyledons became fully green and contained high ATP levels, implicating that a photoheterotrophic state was required to ensure high fluxes. Based on these data, we propose a model for the role of embryonic photosynthesis to improve the energy status of the embryo.