A lysosome-targeted two-photon fluorescence probe for imaging of sulfur dioxide derivatives in living cells and zebrafish
A lysosome-targeted two-photon fluorescence probe for imaging of sulfur dioxide derivatives in living cells and zebrafish
复制标题
用于活细胞和斑马鱼中二氧化硫衍生物成像的溶酶体靶向双光子荧光探针
DOI：
10.1016/j.snb.2018.04.069
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Lin Weiying
中科院分区：
文献类型：
--
作者：
Zhao Yuping;Ma Yanyan;Lin Weiying
二氧化硫（SO2）是最危险的大气污染物之一，与多种呼吸系统和神经系统疾病有关。同时，溶酶体作为活细胞的清道夫，在代谢过程中起着举足轻重的作用。然而，二氧化硫在亚细胞水平上的病理机制仍然是难以捉摸的，部分原因是缺乏检测SO2在细胞器中的方法。因此，对溶酶体中SO2的研究具有重要意义。本文基于独特的C双键N异构化机制，开发了一种用于检测SO2衍生物的双光子荧光探针（Na-SO2-Lyso）。Na-SO_2-Lyso探针对SO_2的响应时间小于15 s，灵敏度高。首次将Na-SO_2-Lyso有效地应用于活细胞溶酶体中SO_2的显像。Na-SO_2-Lyso也可用于检测活体斑马鱼体内SO_2衍生物。
Sulfur dioxide (SO2), as one of the most dangerous atmospheric pollutants, is associated with several respiratory and neurological diseases. Meanwhile, lysosomes, as scavenger of living cells, play a pivotal role in the metabolic process. However, the pathologic mechanisms of sulfur dioxide at the subcellular level remain elusive in part due to the lack of methods for detecting of SO2in the organelles. Accordingly, the study of SO2in lysosome is of high interest. Herein, a two-photon fluorescence probe (Na-SO2-Lyso) for detecting of SO2derivatives was developed based on a unique Cdouble bondN isomerization mechanism. The probeNa-SO2-Lysocould respond to SO2rapidly in less than 15 s with excellent sensitivity. Significantly,Na-SO2-Lysowas effectively applied to image of SO2in lysosomes of living cells for the first time. Moreover,Na-SO2-Lysoalso can be used for detecting of SO2derivatives in living zebrafish.