Individually resolved DNA molecules stretched and embedded in electrospun polymer nanofibers
Individually resolved DNA molecules stretched and embedded in electrospun polymer nanofibers
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DOI：
10.1021/nl061894
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发表时间：
2006-11-08
期刊：
影响因子：
10.8
通讯作者：
Craighead, H. G.
中科院分区：
文献类型：
--
作者：
Bellan, Leon M.;Cross, Joshua D.;Craighead, H. G.
我们利用静电纺丝射流的流动特性来延长和固定 DNA 分子。我们将荧光标记的 lambda 噬菌体 DNA 分子嵌入聚环氧乙烷纳米纤维中并进行观察。使用荧光显微镜对嵌入的 DNA 分子进行成像，发现其拉伸至接近完全染色轮廓长度的长度。
We have used the flow characteristics of an electrospinning jet to elongate and fix DNA molecules. We embedded and observed fluorescently labeled lambda bacteriophage DNA molecules in polyethylene oxide nanofibers. The embedded DNA molecules were imaged using fluorescence microscopy and found to be stretched to lengths approaching the full dyed contour length.