Zeb1-mediated T-cadherin repression increases the invasive potential of gallbladder cancer
Zeb1-mediated T-cadherin repression increases the invasive potential of gallbladder cancer
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DOI：
10.1016/j.febslet.2008.12.042
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发表时间：
2009-01-22
期刊：
影响因子：
3.5
通讯作者：
Furihata, Mutsuo
中科院分区：
文献类型：
--
作者：
Adachi, Yoshihiro;Takeuchi, Tamotsu;Furihata, Mutsuo
在这里，我们报告说，转录调节Zeb 1抑制T-钙粘蛋白的转录，增加胆囊癌细胞的侵袭活性。Zeb 1物理绑定到T-钙粘蛋白的启动子，抑制启动子活性的E盒样序列依赖的方式，并抑制T-钙粘蛋白的表达。在胆囊癌组织中，Zeb 1在癌浸润前沿表达，而T-cadherin仅在非浸润性病灶中表达。胶原凝胶侵袭实验显示T-cadherin是胆囊癌侵袭的负调节因子。这些发现表明，Zeb 1抑制T-cadherin的表达，从而增加胆囊癌的侵袭活性。(C)2008年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Here, we report that the transcriptional regulator Zeb1 repressed the transcription of T-cadherin, to increase the invasive activity of gallbladder cancer cells. Zeb1 physically bound to the promoter of T-cadherin, repressed promoter activity in E-box-like sequence-dependent fashion, and suppressed T-cadherin expression. In gallbladder cancer tissues, Zeb1 was expressed at the cancer invasion front, whereas T-cadherin was exclusively expressed in non-invasive foci. Collagen gel invasion assay showed that T-cadherin was a negative regulator for gallbladder cancer invasion. These findings suggest that Zeb1 represses T-cadherin expression and thus increases the invasive activity of gallbladder cancer. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.