Thioredoxin h System and Wheat Seed Quality
Thioredoxin h System and Wheat Seed Quality
复制标题
DOI：
10.1094/cchem-85-6-0799
复制
发表时间：
2008-11
期刊：
影响因子：
2.4
通讯作者：
Abderrakib Zahid;Samia Afoulous;R. Cazalis
中科院分区：
文献类型：
--
作者：
Abderrakib Zahid;Samia Afoulous;R. Cazalis
硫氧还蛋白是生物体内控制细胞氧化还原平衡的关键系统之一。植物硫氧还蛋白是一个多基因家族，分为叶绿体和细胞质两个系统，这两个系统可以通过电子供体和催化硫氧还蛋白还原的酶来区分。在谷物种子中，硫氧还蛋白（Trx）h系统在发育阶段起作用，控制种子灌浆期间化合物的递送。在吸胀种子发育的早期，它通过抑制淀粉分解酶的灭活剂和通过激活特定的丝氨酸蛋白酶硫代钙蛋白酶来促进胚乳中储存氮和碳的动员。在种子成熟和萌发过程中，Trx h系统控制活组织中的氧化应激，特别是在盾片和糊粉层中，在那里它积累在细胞核中。Trx的过表达和抑制证实了这些特征，并构成了管理种子质量的有力工具。
ABSTRACT Thioredoxin is one of the key systems controlling cellular redox balance in all living organisms. Plant thioredoxins are a diverse multigene family divided into two systems, the chloroplastic and the cytoplasmic systems, which are distinguishable by the electron donor and by the enzyme that catalyses thioredoxin reduction. In cereal seed, the thioredoxin (Trx) h system acts in the developing phase, controlling the delivery of compounds during seed filling. Early in the development of the imbibed seed, it promotes the mobilization of storage nitrogen and carbon in the endosperm by inhibiting the inactivators of amylolytic enzyme and by activating a specific serine protease, thiocalsin. During seed maturation and germination, the Trx h system controls oxidative stress in the living tissues, specifically in the scutellum and the aleurone layer, where it accumulates in the nucleus. The overexpression and the suppression of Trx confirm these features and constitute a powerful tool to manage seed quali...