Delivery of exogenous protein antigens to major histocompatibility complex class I pathway in cytosol
Delivery of exogenous protein antigens to major histocompatibility complex class I pathway in cytosol
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DOI：
10.1086/338448
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发表时间：
2002-01-15
影响因子：
6.4
通讯作者：
Lu, YC
中科院分区：
文献类型：
--
作者：
Cao, HY;Agrawal, D;Lu, YC
炭疽致死因子的片段具有通过经典的主要组织相容性复合物 (MHC) I 类途径递送重组蛋白抗原的有趣功能。致死因子的这个区域缺乏与炭疽细胞毒性相关的结构域，并且独立于其二元伙伴保护性抗原发挥作用。在 MHC I 类途径的不同步骤使用抑制剂的实验支持了这一假设。将这一发现应用到当前的 T 细胞测定中，无需借助活载体即可测量细胞毒性 T 淋巴细胞功能，并为设计和测试 T 细胞依赖性疫苗提供了有用的新工具。
A fragment of anthrax lethal factor possesses the interesting function of delivering recombinant protein antigens through the classical major histocompatibility complex (MHC) class I pathway. This region of the lethal factor lacks the domain associated with anthrax cytotoxicity and functions independently of its binary partner, protective antigen. Experiments that used inhibitors at different steps of the MHC class I pathway supported this hypothesis. Application of this discovery to current T cell assays allows for the measurement of cytotoxic T lymphocyte function without resorting to live vectors and provides a useful new tool to design and test T cell-dependent vaccines.