Transcriptomic profiling and functional prediction reveal aberrant expression of circular RNAs during osteogenic differentiation in human umbilical cord mesenchymal stromal cells
Transcriptomic profiling and functional prediction reveal aberrant expression of circular RNAs during osteogenic differentiation in human umbilical cord mesenchymal stromal cells
复制标题
DOI：
10.1038/s41598-021-98470-2
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Wenchuan Chen
中科院分区：
文献类型：
--
作者：
Cheng Su;Xiao Zheng;Yanjin He;Li Long;Wenchuan Chen
Circular RNAs (circRNAs) are crucial elements of non-coding RNA, that regulate various biological processes. To date, expression patterns and functional roles .of circRNAs during osteogenic differentiation of human umbilical cord mesenchymal stromal cells (hUCMSCs) remain unknown. In this study, we analyzed RNA-sequence data to reveal expression profiles of circRNAs during osteogenesis of hUCMSCs, then elucidated the underlying mechanisms of action. We identified a total of 5457 circRNAs in hUCMSCs, of which 34 and 33 were upregulated and downregulated, respectively. We applied Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses to determine functions and related pathways of differentially expressed circRNAs. Moreover, we applied bioinformatics tools to construct competing endogenous RNA networks, comprising 10 circRNAs, 46 micro RNAs (miRNAs) and 413 mRNAs. Furthermore, we predicted protein-coding circ‐CTTN could promote osteogenesis of hUCMSCs. Overall, our findings indicate that clusters of circRNAs are aberrantly expressed in hUCMSCs during osteogenic differentiation, hence lay a foundation for future research into promoting hUCMSCs osteogenic differentiation and bone regeneration potential of the upregulated circRNAs then constructed a co-expression network .comprising the top 5 upregulated circRNAs and 75 RNA-binding proteins (RBPs). .Next, we validated 6 differentially-expressed circRNAs and found that overexpressing
