Cultured primary human fetal retinal pigment epithelium (hfRPE) as a model for evaluating RPE metabolism.
Cultured primary human fetal retinal pigment epithelium (hfRPE) as a model for evaluating RPE metabolism.
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DOI：
10.1016/j.exer.2014.01.015
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发表时间：
2014-09
影响因子：
3.4
通讯作者：
Philp, Nancy J.
中科院分区：
文献类型：
--
作者：
Adijanto, Jeffrey;Philp, Nancy J.
线粒体功能障碍已被证明会导致与年龄相关的增殖性视网膜疾病。在过去的十年中，原代人胎儿RPE（hfRPE）培养模型已成为研究RPE功能和视网膜疾病机制的有效工具。该模型系统经过严格表征，显示在基因组和蛋白质水平上与天然 RPE 细胞非常相似，并且它们能够实现健康天然 RPE 的特征功能（例如，视杆吞噬作用、离子和液体运输以及类视黄醇循环）。在这篇综述中，我们证明了 RPE 的代谢活性是其健康和分化状态的指标，并将 hfRPE 培养模型作为一种有价值的体外系统，用于评估 RPE 分化和视网膜疾病背景下的 RPE 代谢。
Mitochondrial dysfunction has been shown to contribute to age-related and proliferative retinal diseases. Over the past decade, the primary human fetal RPE (hfRPE) culture model has emerged as an effective tool for studying RPE function and mechanisms of retinal diseases. This model system has been rigorously characterized and shown to closely resemble native RPE cells at the genomic and protein level, and that they are capable of accomplishing the characteristic functions of a healthy native RPE (e.g., rod phagocytosis, ion and fluid transport, and retinoid cycle). In this review, we demonstrated that the metabolic activity of the RPE is an indicator of its health and state of differentiation, and present the hfRPE culture model as a valuable in vitro system for evaluating RPE metabolism in the context of RPE differentiation and retinal disease.
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作者：
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DOI：
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