A novel plant defensin-like gene of winter wheat is specifically induced during cold acclimation
A novel plant defensin-like gene of winter wheat is specifically induced during cold acclimation
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DOI：
10.1016/s0006-291x(02)02391-4
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发表时间：
2002-10-18
影响因子：
3.1
通讯作者：
Imai, R
中科院分区：
文献类型：
--
作者：
Koike, M;Okamoto, T;Imai, R
通过对冷锻炼诱导基因的差异筛选，从冬小麦冠层组织中克隆到一个新的cDNA克隆Tad 1。Tad 1 cDNA编码一个23 kDa的多肽，具有潜在的N-末端信号序列。推定的成熟序列显示出惊人的相似性，植物防御素或γ-硫素，代表低分子大小的抗病多肽。高水平的Tad 1 mRNA积累发生在一天的冷驯化冠组织和水平保持整个14天的冷驯化。在低温处理的幼苗中观察到类似的快速诱导，而外源脱落酸则没有。与来自其他植物物种的防御素相反，水杨酸和茉莉酸甲酯均不诱导Tad 1的表达。TAD 1多肽的重组成熟形式抑制植物病原细菌菊苣假单胞菌的生长，然而，没有检测到抗冻活性。总的来说，这些数据表明，Tad 1诱导在冷驯化的冬小麦独立的主要防御信号（S），并参与低温诱导的抗病性，在冬季锻炼的病原体。(C)2002 Elsevier Science（美国）。All rights reserved.
A novel cDNA clone, Tad1, was isolated from crown tissue of winter wheat after differential screening of cold acclimation-induced genes. The Tad1 cDNA encoded a 23 kDa polypeptide with a potential N-terminal signal sequence. The putative mature sequence showed striking similarity to plant defensins or gamma-thionins, representing low molecular size antipathogenic polypeptides. High levels of Tad1 mRNA accumulation occurred within one day of cold acclimation in crown tissue and the level was maintained throughout 14 days of cold acclimation. Similar rapid induction was observed in young seedlings treated with low temperature but not with exogenous abscisic acid. In contrast to defensins from other plant species, neither salicylic acid nor methyl jasmonate induced expression of Tad1. The recombinant mature form of TAD1 polypeptide inhibited the growth of the phytopathogenic bacteria, Pseudomonas cichorii; however, no antifreeze activity was detected. Collectively, these data suggested that Tad1 is induced in cold-acclimated winter wheat independent of major defense signaling(s) and is involved in low temperature-induced resistance to pathogens during winter hardening. (C) 2002 Elsevier Science (USA). All rights reserved.