Three-Dimensional pH Microprobing with an Optically-Manipulated Fluorescent Particle

Three-Dimensional pH Microprobing with an Optically-Manipulated Fluorescent Particle
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DOI:
10.1246/cl.1996.141
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发表时间:
1996-02
期刊:
影响因子:
1.6
通讯作者:
K. Sasaki;Z. Shi;R. Kopelman;H. Masuhara
K. Sasaki;Z. Shi;R. Kopelman;H. Masuhara
中科院分区:
化学4区
文献类型:
--
作者:
K. Sasaki;Z. Shi;R. Kopelman;H. Masuhara

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研制了一种新型的pH微探针,可以用亚微米级的三维分辨率测量不均匀的pH分布。用激光捕获技术对掺入pH敏感荧光素-丙烯酰胺的聚丙烯酰胺颗粒进行了操纵。结果表明,水/玻璃界面的局部pH不同于整体水的局部pH。
A novel pH microprobe has been developed, which makes it possible to measure inhomogeneous pH distributions with submicrometer three-dimensional resolution. A polyacrylamide particle incorporated with pH-sensitive fluorescein-acrylamide is manipulated with a laser trapping technique. It is demonstrated that the local pH in a water/glass interface is different from that of bulk water.