The role of iron-induced hippocampal peroxidation in acute epileptogenesis
The role of iron-induced hippocampal peroxidation in acute epileptogenesis
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DOI：
10.1016/0006-8993(86)91356-9
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发表时间：
1986-09
期刊：
影响因子：
2.9
通讯作者：
L. Willmore;W. Triggs;J. Gray
中科院分区：
文献类型：
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作者：
L. Willmore;W. Triggs;J. Gray
皮质内注射铁盐可引起脂质过氧化、局灶性水肿、坏死、神经胶质增生，并可发展成癫痫发作。生育酚预处理可防止与铁注射相关的组织病理学扰动，并似乎加速了过氧化产物局部蓄积的消退。在本实验中，大鼠在背侧海马注射3 μl 100 mM FeCl 2之前，先用500 mg/kgDL-α-生育酚乙酸酯预处理，或通过s.c.注射0.8mg/100 g荷包牡丹素。生育酚预处理防止了大量铁盐注射动物惊厥发作的发生。在未处理的大鼠铁诱导癫痫发作和生育酚处理的大鼠，但发展惊厥性癫痫发作的解剖海马中测得的脂质过氧化显着增加。生育酚未能防止荷包牡丹素诱导的癫痫发作。此外，荷包牡丹素诱导的惊厥发作未能改变海马荧光水平。因此，我们得出结论，海马注射铁盐的致癫痫作用似乎与注射部位内神经脂质过氧化的诱导有关。
Intracortical injection of iron salts causes lipid peroxidation, focal edema, necrosis, gliosis, and the development of bahavioral and electrographic seizures. Tocopherol pretreatment prevents the histopathologic pertubations associated with iron injection, and appears to accelerate the resolution of focal accumulation of peroxidation products. In this experiment, rats were pretreated with 500 mg/kgDL-α-tocopherol acetate prior to the injection of 3 μl of 100 mM FeCl2into the dorsal hippocampus, or induction of convulsive seizures by s.c. injection of 0.8 mg/100 g bicucullin. Tocopherol pretreatment prevented the occurrence of convulsive seizures in a significant number of iron-salts injected animals. Lipid peroxidation measured in the dissected hippocampus was significantly increased in untreated rats developing iron-induced seizures and in rats treated with tocopherol, but developing convulsive seizures. Tocopherol failed to prevent bicucullin-induced seizures. Further, convulsive seizures induced by bicucullin failed to alter hippocampal fluorescence levels. Hence, we concluded that the epileptogenic effects of hippocampal injection of iron salts appear to be related to the induction of peroxidation of neural lipids within the injection site.