Riboregulation in Escherichia coli:: DsrA RNA acts by RNA:RNA interactions at multiple loci
Riboregulation in Escherichia coli:: DsrA RNA acts by RNA:RNA interactions at multiple loci
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DOI：
10.1073/pnas.95.21.12456
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发表时间：
1998-10-13
影响因子：
11.1
通讯作者：
Belfort, M
中科院分区：
文献类型：
--
作者：
Lease, RA;Cusick, ME;Belfort, M
DSRA是一个87个核苷酸的非翻译核糖核酸，它既调节全球转录沉默蛋白和类核蛋白II-NS，又调节稳定期和应激反应sigma因子rpos(sigma(S))。我们证明DSRA通过HNS基因座上特定的RNA：RNA碱基配对相互作用来拮抗H-NS翻译。我们还给出了证据，支持RNA：rpos位点的RNA相互作用促进rpos翻译。DSRA对HNS的负调控是通过RNA：RNA相互作用阻止HNS RNA的翻译实现的，相反，结果表明DSRA对RPOS的正调控是通过形成激活顺式作用的翻译操纵子的RNA结构来实现的。DSRA内的序列与另外三个基因argR、ilvIH和rbsD互补，表明DSRA是基因表达的核转录调节因子，通过多个位点的RNA：RNA相互作用来协调作用。
DsrA is an 87-nt untranslated RNA that regulates both the global transcriptional silencer and nucleoid protein II-NS and the stationary phase and stress response sigma factor RpoS (sigma(s)). We demonstrate that DsrA acts via specific RNA:RNA base pairing interactions at the hns locus to antagonize H-NS translation. We also give evidence that supports a role for RNA:RNA interactions at the rpoS locus to enhance RpoS translation. Negative regulation of hns by DsrA is achieved by the RNA:RNA interaction blocking translation of hns RNA, In contrast, results suggest that positive regulation of rpoS by DsrA occurs by formation of an RNA structure that activates a cis-acting translational operator. Sequences within DsrA complementary to three additional genes, argR, ilvIH, and rbsD, suggest that DsrA is a riboregulator of gene expression that acts coordinately via RNA:RNA interactions at multiple loci.