A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.
A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.
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DOI：
10.1007/978-1-4939-6545-8_9
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yu HG
中科院分区：
文献类型：
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作者：
Fan J;Jin H;Yu HG
在本章中，我们使用报告绿色荧光蛋白（GFP）与内部红色荧光蛋白（RFP）对照的比例描述了一种基于荧光的基因表达定量分析。通过这种双色异源报告基因分析，我们发现了粘合蛋白调控基因，并发现了一个顺式作用的DNA元件Ty1-LTR，它与粘合蛋白相互作用，并在酵母减数分裂过程中调控基因表达。本方法为芽殖酵母减数分裂过程中基因表达的定量分析提供了一种有效的细胞学方法。
In this chapter, we describe a quantitative fluorescence-based assay of gene expression using the ratio of the reporter green fluorescence protein (GFP) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes and discovered a cis-acting DNA element, the Ty1-LTR, which interacts with cohesin and regulates gene expression during yeast meiosis. The method described here provides an effective cytological approach for quantitative analysis of global gene expression in budding yeast meiosis.