Hydroxyl radical scavenging mechanism of human erythrocytes by quercetin-germanium (IV) complex.
Hydroxyl radical scavenging mechanism of human erythrocytes by quercetin-germanium (IV) complex.
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DOI：
10.1016/j.ejps.2012.04.019
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发表时间：
2012-08
期刊：
影响因子：
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通讯作者：
Shengguang Li;Wei-Ling Xie;Huai-hong Cai;Ji-Ye Cai;Pei-Hui Yang
中科院分区：
文献类型：
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作者：
Shengguang Li;Wei-Ling Xie;Huai-hong Cai;Ji-Ye Cai;Pei-Hui Yang
槲皮素是一种常见的黄酮类化合物，存在于植物食品、草药和膳食补充剂中。锗是一种微量元素，具有增强机体免疫力的作用。本研究探讨了槲皮素锗（Quercertin-Ge（IV））配合物对人红细胞羟自由基的清除机制，特别是对红细胞膜超微结构和机械性质、细胞膜电位和细胞内游离Ca ~（2+）浓度的影响。结果表明，Qu-Ge配合物QuGe 2能减轻红细胞的氧化损伤，改变细胞表面形态，部分恢复细胞膜电位和胞内游离Ca ~（2+）水平。利用原子力显微镜（AFM）在纳米尺度上观察细胞形态、细胞膜超微结构和生物物理特性的变化。Qu Ge 2可触发抗氧化因子抑制细胞损伤。这些结果有助于进一步了解曲锗配合物对人红细胞羟自由基的清除机制，并有望开发成为一种新的抗氧化剂，用于治疗氧化损伤。
Quercetin is a popular flavonoid in plant foods, herbs, and dietary supplement. Germanium, a kind of trace elements, can enhance the body immunity. This study investigated the hydroxyl-radical-scavenging mechanism of the quercertin–germanium (IV) (Qu–Ge) complex to human erythrocytes, especially the effects on ultrastructure and mechanical properties of cell membrane, plasma membrane potential and intracellular free Ca2+concentration. Results showed that QuGe2, a kind of the Qu–Ge complex, could reduce the oxidative damage of erythrocytes, change the cell-surface morphology, and partly recover the disruption of plasma membrane potential and intracellular free Ca2+level. Atomic force microscopy (AFM) was used to characterize the changes of the cell morphology, cell-membrane ultrastructure and biophysical properties at nanoscalar level. QuGe2has triggered the antioxidative factor to inhibit cellular damage. These results can improve the understanding of hydroxyl-radical-scavenging mechanism of human erythrocytes induced by the Qu–Ge complex, which can be potentially developed as a new antioxidant for treatment of oxidative damage.