Live Imaging of Tardigrade Embryonic Development by Differential Interference Contrast Microscopy
Live Imaging of Tardigrade Embryonic Development by Differential Interference Contrast Microscopy
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微分干涉对比显微镜对缓步动物胚胎发育的实时成像
DOI：
10.1101/pdb.prot102335
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发表时间：
2018
影响因子：
--
通讯作者：
Goldstein, Bob
中科院分区：
文献类型：
--
作者：
Heikes, Kira L.;Goldstein, Bob
选择缓步动物 Hypsibius exemplaris 作为模型系统，部分原因是胚胎和动物在所有阶段都是光学透明的，有利于观察和拍摄内部过程。微分干涉（DIC）显微镜下的多平面视频记录允许通过七轮分裂重建早期胚胎细胞谱系，并揭示了不对称细胞分裂、核迁移和细胞迁移的不变模式。在这里，我们提出了通过 DIC 显微镜拍摄 H. exemplaris 胚胎发育的协议。
The tardigrade Hypsibius exemplaris was chosen as a model system in part because embryos and animals are optically clear at all stages, facilitating the viewing and filming of internal processes. Multiplane video recordings under differential interference contrast (DIC) microscopy have allowed early embryonic cell lineages to be reconstructed through seven rounds of division and have revealed invariant patterns of asymmetric cell divisions, nuclear migrations, and cell migrations. Here, we present a protocol for filming embryonic development of H. exemplaris by DIC microscopy.