Expression Profiles of Oncomir miR-21 and Tumor Suppressor let-7a in the Progression of Opisthorchiasis-Associated Cholangiocarcinoma
Expression Profiles of Oncomir miR-21 and Tumor Suppressor let-7a in the Progression of Opisthorchiasis-Associated Cholangiocarcinoma
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DOI：
10.7314/apjcp.2012.13.kksuppl.65
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发表时间：
2012-01-01
影响因子：
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通讯作者：
Yongvanit, P.
中科院分区：
文献类型：
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作者：
Namwat, N.;Chusorn, P.;Yongvanit, P.
MiRNA表达改变可能是癌症发生和/或进展的决定因素。本研究旨在探讨oncomiR-21和抑癌基因let-7a在尾棘皮病相关胆管细胞癌(CCA)发生中的作用。结果表明，在金黄地鼠胆管癌变过程中，miR-21基因表达上调，let-7a基因表达下调。MiR-21在人CCA中的表达水平与其靶肿瘤转移抑制基因RECK的表达水平呈负相关。KKU100 CCA细胞miR-21基因敲除后，RECK基因表达水平显著升高，并抑制创伤诱导的CCA细胞迁移。我们的数据表明miR-21是在CCA的生长和转移中起关键作用的一个关键分子。操纵miRNA的表达为CCA治疗提供了一条潜在的途径。
Altered miRNA expression could be a determinant of cancer development and/or progression. We aimed to study the role of oncomir miR-21 and tumor suppressor let-7a in the genesis of Opisthorchiasis-associated cholangiocarcinoma (CCA). The results showed that miR-21 was up-regulated while let-7a was down-regulated during cholangiocarcinogenesis in the hamster model and also in human CCA samples. The expression level of miR-21 had an inverse correlation with the mRNA level of its target RECK, a metastasis suppressor, in human CCA. Knockdown of miR-21 of KKU100 CCA cells significantly increased the mRNA level of RECK and suppressed the wound-induced migration of CCA cells. Our data suggest that miR-21 is one key molecule playing crucial roles in the CCA growth and metastasis. Manipulation of miRNA expression offers a potential avenue of CCA therapy.