Energy-filtered transmission electron microscopy of ferritin.
Energy-filtered transmission electron microscopy of ferritin.
复制标题
铁蛋白的能量过滤透射电子显微镜。
DOI：
10.1073/pnas.79.1.106
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
Somlyo,AP
中科院分区：
文献类型：
--
作者：
Shuman,H;Somlyo,AP
磁扇形谱仪的聚焦特性被证明是适合于形成高空间分辨率，能量过滤的透射电子显微镜图像。通过使用从特征铁M2、3和碳K吸收边缘散射的电子过滤的铁蛋白分子的图像清楚地区分75-A铁核和120-A蛋白质壳。当电子剂量为18 C/cm ~ 2时，Fe的最小可探测质量为0.84 × 10 ~（-20）g，置信度为99%。
The focusing properties of a magnetic-sector spectrometer are shown to be suitable for forming high-spatial-resolution, energy-filtered transmission electron microscope images. Filtered images of ferritin molecules by using electrons scattered from the characteristic iron M2,3 and carbon K absorption edges clearly distinguish the 75-A iron core and 120-A protein shell. The minimum detectable mass is estimated to be 0.84 X 10(-20) g for Fe for an electron dose of 18 C/cm2 and 99% confidence.