Detection of a mammalian histone H4 kinase that has yeast histidine kinase-like enzymic activity
Detection of a mammalian histone H4 kinase that has yeast histidine kinase-like enzymic activity
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DOI：
10.1016/s1357-2725(99)00119-3
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发表时间：
2000-02-01
影响因子：
4
通讯作者：
Attwood, PV
中科院分区：
文献类型：
--
作者：
Besant, PG;Attwood, PV
从酵母中提纯的一种具有良好特性的组氨酸激酶已被证明能将组蛋白H4磷酸化在组氨酸残基上。这种酶不同于主要在原核生物中发现的双组分组氨酸激酶。到目前为止，还没有从哺乳动物中纯化出类似于这种酵母酶的组氨酸激酶。通过使用与纯化酵母组氨酸激酶类似的纯化方案，从猪胸腺中分离出了具有组蛋白H4激酶活性的蛋白质组分。用凝胶内酶检测系统可以检测到酵母组氨酸激酶，利用该系统，猪胸腺制剂中有四条主要的组蛋白H4酶活性条带。通过免疫沉淀、碱性水解和随后的磷酸氨基酸分析，证明了该部分纯化的激酶部分能够将组蛋白H4磷酸化到组氨酸上。总之，已经从猪胸腺中制备了一种含有组蛋白H4激酶活性的制剂，该制剂中至少存在一种组氨酸激酶。(C)2000爱思唯尔科学有限公司。保留所有权利。
A well characterized histidine kinase purified from yeast has been shown to phosphorylate histone H4 on a histidine residue. This enzyme is unlike the two-component histidine kinases predominantly found in prokaryotes. Until now, a histidine kinase similar to this yeast enzyme has not been purified from a mammalian source.By using a purification scheme similar to that used to purify the yeast histidine kinase, a protein fraction with histone H4 kinase activity has been isolated from porcine thymus. The yeast histidine kinase was shown to be detectable using an in-gel kinase assay system and using this system, four major bands of histone H4 kinase activity were apparent in the porcine thymus preparation. Through the use of immunoprecipitation, alkaline hydrolysis and subsequent phosphoamino acid analysis it has been demonstrated that this partially purified kinase fraction is capable of phosphorylating histone H4 on histidine. In conclusion, an preparation has been made from porcine thymus that contains histone H4 kinase activity and at least one of the kinases present in this preparation is a histidine kinase. (C) 2000 Elsevier Science Ltd. All rights reserved.