Electrophoretic analysis of the prey spectrum of Teretrius nigrescens (Lewis) (Col., Histeridae), a predator of Prostephanus truncatus (Horn) (Col., Bostrichidae), in Mexico, Honduras, and Benin
Electrophoretic analysis of the prey spectrum of Teretrius nigrescens (Lewis) (Col., Histeridae), a predator of Prostephanus truncatus (Horn) (Col., Bostrichidae), in Mexico, Honduras, and Benin
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DOI：
10.1046/j.1439-0418.2003.00772.x
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发表时间：
2003-07-01
影响因子：
1.9
通讯作者：
Poehling, HM
中科院分区：
文献类型：
--
作者：
Camara, M;Borgemeister, C;Poehling, HM
在墨西哥，洪都拉斯和贝宁的Terretrius nigrescens的广泛收集，进行了详细的分析，以阐明捕食者的猎物谱。聚丙烯酰胺凝胶电泳和醋酸纤维素电泳。甲虫采样与信息素陷阱，使用合成的聚集信息素的截形前翅虫，并直接从农民的玉米商店。成虫中可检测到的猎物蛋白的比例。在1108个样本中，只有34个样本的猎物蛋白可以被清楚地识别出来。更常见的是，猎物蛋白检测到成年T。nigrescens在墨西哥，洪都拉斯和贝宁的玉米商店采样，87个样本显示不同的猎物带的总1214捕食者分析。在241 T。nigrescens幼虫在贝宁的玉米仓库中取样，136显示出明显的猎物蛋白带。在所有的样本中，截形拟步行虫是最常见的被捕食物种。第二个最常见的被捕食物种是玉米象。结果进行了讨论方面的各种方法的猎物光谱分析，特别是生物学的T。黑色的
Extensive collections of Teretrius nigrescens in Mexico, Honduras, and Benin, were electrophoretically analysed to elucidate the prey spectrum of the predator. Both polyacrylamide gel electrophoresis and cellulose acetate electrophoresis were used. Beetles were sampled with pheromone traps, using the synthetic aggregation pheromone of Prostephanus truncatus, and directly from farmers' maize stores. The proportion of electrophoretically detected prey protein from adult T. nigrescens in pheromone traps was low: of the 1108 specimens analysed, only in 34 cases, prey protein could be clearly identified. More frequently, prey protein was detected in adult T. nigrescens sampled in maize stores in Mexico, Honduras, and Benin, with 87 samples showing distinct prey bands of the total 1214 predators analysed. Of the 241 T. nigrescens larvae sampled in maize stores in Benin, 136 showed distinct bands of prey protein. In all samples, P. truncatus was the most frequently detected prey species. The second most often identified prey species was Sitophilus zeamais. The results are discussed with regard to various methods for prey spectrum analysis and specifically the biology of T. nigrescens.