Effectiveness of Radiofrequency Hyperthermia for Treating Cartilage in Guinea Pigs with Primary Osteoarthritis
Effectiveness of Radiofrequency Hyperthermia for Treating Cartilage in Guinea Pigs with Primary Osteoarthritis
复制标题
DOI：
10.1177/1947603516678974
复制
发表时间：
2018
期刊：
影响因子：
2.8
通讯作者：
Kenji A Takahashi;Hiroshi Nakamura;H. Ozawa;S. Hashimoto;N. Iijima;Shimpei Higo;H. Watanabe;Y. Mochizuki;S. Takai
中科院分区：
文献类型：
--
作者：
Kenji A Takahashi;Hiroshi Nakamura;H. Ozawa;S. Hashimoto;N. Iijima;Shimpei Higo;H. Watanabe;Y. Mochizuki;S. Takai
目的自噬是维持软骨细胞功能的重要因素，自噬减少导致骨关节炎（OA）的发生。先前的研究表明热休克应激参与了细胞自噬的控制。本研究旨在研究高温对Hartley豚鼠关节软骨中自噬相关蛋白表达和自然发生的OA进展的影响。将13.56 MHz的设计辐射脉冲施加到动物的膝盖上20分钟以诱导体温过高。分别于热疗后8h、24 h、72 h、7 d、6个月切除膝关节。对膝关节连续切片进行组织病理学检查。免疫组化法检测Unc-51样激酶1（ULK 1）和Beclin 1的表达水平。结果ULK 1和Beclin 1在中度OA软骨中的表达明显低于正常软骨（P <0.01），在中度OA软骨中ULK 1和Beclin 1的表达明显低于正常软骨（P < 0.01）。暴露于射频波后7天，ULK 1和Beclin 1的表达水平在轻度OA动物的SZ中增加。与未治疗膝关节相比，射频治疗膝关节的软骨退化严重程度显著降低（P < 0.01）。结论热刺激可促进正常膝关节软骨细胞和轻度OA膝关节软骨细胞的自噬。该研究还表明，长期定期应用高温可抑制与衰老相关的OA进展。射频热疗激活自噬可能是治疗骨关节炎的一种有效方法。
Objective Autophagy was reported to be essential for maintaining chondrocyte function, and reduced autophagy leads to osteoarthritis (OA). Previous studies showed involvement of heat shock stress in the control of autophagy in cells. This study sought to investigate the effect of hyperthermia on the expression of autophagy-related proteins in articular cartilage and the progression of naturally occurring OA in Hartley guinea pigs. Design Radiofrequency pulses of 13.56 MHz were applied to the animals’ knees for 20 minutes to induce hyperthermia. The knee joints were resected at 8 hours, 24 hours, 72 hours, 7 days, and 6 months after hyperthermia. Serial sections of knees were examined for histopathological changes. The expression levels of Unc-51-like kinase 1 (ULK1) and Beclin1 were analyzed by immunohistochemistry. Results Analysis of the distribution of positive cells showed that, in cases of moderate OA, ULK1 and Beclin1 expression levels were significantly decreased in the superficial zone (SZ) and middle zone (MZ) (P < 0.01) compared with normal cartilage. Seven days after exposure to radiofrequency waves, expression levels of ULK1 and Beclin1 were augmented in the SZ in animals with mild OA. The severity of cartilage degradation was significantly reduced (P < 0.01) in the radiofrequency-treated knees versus the untreated knees. Conclusions This study showed that heat stimulation enhanced autophagy in healthy knee chondrocytes and chondrocytes in knees with mild OA. The study also showed that long-term periodic application of hyperthermia suppresses aging-related progression of OA. The activation of autophagy by radiofrequency hyperthermia may be an effective therapeutic approach for osteoarthritis.