Bacterial Communities Associated with the Digestive Tract of the Predatory Ground Beetle, Poecilus chalcites, and Their Modification by Laboratory Rearing and Antibiotic Treatment
Bacterial Communities Associated with the Digestive Tract of the Predatory Ground Beetle, Poecilus chalcites, and Their Modification by Laboratory Rearing and Antibiotic Treatment
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DOI：
10.1007/s00248-008-9415-6
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发表时间：
2009-02-01
影响因子：
3.6
通讯作者：
Petzke, Lynn M.
中科院分区：
文献类型：
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作者：
Lehman, R. Michael;Lundgren, Jonathan G.;Petzke, Lynn M.
土甲虫（鞘翅目：步甲科）等是农业系统中的益虫，它们有助于控制害虫和杂草。我们利用聚合酶链反应扩增的16S rRNA基因的末端限制性片段长度多态性分析，评估了野外采集的P. chaltes消化道中细菌群落的复杂性。通过构建16S rRNA基因克隆文库和序列分析进行细菌鉴定。然后将野外采集的甲虫的肠道细菌与实验室中饲养的有抗生素和不含抗生素的人工饲料的甲虫的肠道细菌进行比较。直接细胞计数估计每毫升肠道1.5 × 10(8)个细菌。野外采集的白蚁消化道平均每只产生4.8个末端限制性片段（tRF）。克隆数量最多的是乳杆菌属，其次是肠杆菌科、梭状芽胞杆菌属和拟杆菌属。大部分序列与其他昆虫胃肠道中已报道的序列密切相关。与野外饲养的甲虫相比，实验室饲养的甲虫产生的tRF较少，平均每只甲虫产生3.1个tRF，并且肠杆菌科的克隆数量较多的分类群数量减少。抗生素处理显著（p < 0.05）降低了每只甲虫的tRF数量，并选择了较少多样性的细菌分类群。我们的结论是，P. chalite的消化道是由一个简单的群落细菌定植具有本地特征。实验室饲养的甲虫携带着在野外采集的甲虫中发现的最常见的细菌，这些细菌群落可以在实验室中通过在饮食中添加抗生素来控制，以便研究其功能作用。
Ground beetles such as Poecilus chalcites (Coleoptera: Carabidae) are beneficial insects in agricultural systems where they contribute to the control of insect and weed pests. We assessed the complexity of bacterial communities occurring in the digestive tracts of field-collected P. chalcites using terminal restriction fragment length polymorphism analyses of polymerase chain reaction-amplified 16S rRNA genes. Bacterial identification was performed by the construction of 16S rRNA gene clone libraries and sequence analysis. Intestinal bacteria in field-collected beetles were then compared to those from groups of beetles that were reared in the lab on an artificial diet with and without antibiotics. Direct cell counts estimated 1.5 x 10(8) bacteria per milliliter of gut. The digestive tract of field-collected P. chalcites produced an average of 4.8 terminal restriction fragments (tRF) for each beetle. The most abundant clones were affiliated with the genus Lactobacillus, followed by the taxa Enterobacteriaceae, Clostridia, and Bacteriodetes. The majority of the sequences recovered were closely related to those reported from other insect gastrointestinal tracts. Lab-reared beetles produced fewer tRF, an average of 3.1 per beetle, and a reduced number of taxa with a higher number of clones from the family Enterobacteriaceae compared to the field-collected beetles. Antibiotic treatment significantly (p < 0.05) reduced the number of tRF per beetle and selected for a less diverse set of bacterial taxa. We conclude that the digestive tract of P. chalcites is colonized by a simple community of bacteria that possess autochthonous characteristics. Laboratory-reared beetles harbored the most common bacteria found in field-collected beetles, and these bacterial communities may be manipulated in the laboratory with the addition of antibiotics to the diet to allow study of functional roles.