Developmental stage-specific antigens in the nervous system of the cockroach.
Developmental stage-specific antigens in the nervous system of the cockroach.
复制标题
蟑螂神经系统中发育阶段特异性抗原。
DOI：
10.1016/0012-1606(89)90199-1
复制
发表时间：
1989
影响因子：
2.7
通讯作者：
Marner,JA
中科院分区：
文献类型：
--
作者：
Denburg,JL;Norbeck,BA;Caldwell,RT;Marner,JA
一个具体的努力是获得单克隆抗体，结合大分子发挥作用的神经系统的发展。人们认为，这些分子的良好候选者是那些只短暂存在于胚胎神经系统中的分子。杂交瘤是从取自小鼠的脾细胞制备的，所述小鼠已经用来自43-50%发育阶段的蟑螂胚胎的神经索免疫。筛选杂交瘤上清液中与胚胎和成人胸神经节冷冻切片结合的抗体。保存并克隆产生瞬时结合胚胎神经系统的单克隆抗体的细胞系。这些发育阶段特异性单克隆抗体要么不与成人神经系统结合，要么以与胚胎神经系统非常不同的模式与之结合。由这些单克隆抗体检测到的发育阶段特异性抗原被组织成四类，基于它们瞬时定位的胚胎神经系统的一部分。这些包括与所有神经元的细胞体、神经元或成神经细胞的子集的细胞体、轴突的子集和神经堆的结合。抗原的初步生化表征表明，这些抗体中的许多识别碳水化合物表位。这些抗原的功能，其中大部分是细胞表面的组成部分，暂定。
A specific effort was made to obtain monoclonal antibodies that bind to macromolecules that play a role in the development of the nervous system. It was considered that good candidates for such molecules were those that were only transiently present in the embryonic nervous system. Hybridomas were prepared from spleen cells taken from mice that had been immunized with nerve cords from cockroach embryos at the 43–50% stage of development. The hybridoma supernatants were screened for antibody binding to frozen sections of both embryonic and adult thoracic ganglia. Cell lines that produced monoclonal antibodies that transiently bound to the embryonic nervous system were saved and cloned. These developmental stage-specific monoclonal antibodies either did not bind to the adult nervous system or bound to it with a pattern very different from that in the embryonic nervous system. The developmental stage-specific antigens detected by these monoclonal antibodies were organized into four categories based on the part of the embryonic nervous system in which they were transiently localized. These include binding to the cell bodies of all neurons, cell bodies of subsets of neurons or neuroblasts, subsets of axons, and the neuropile. Preliminary biochemical characterization of the antigens showed that many of these antibodies were recognizing carbohydrate epitopes. Functions for these antigens, most of which are components of the cell surface, are tentatively proposed.