Characterization of biomechanical properties of cells through dielectrophoresis-based cell stretching and actin cytoskeleton modeling.
Characterization of biomechanical properties of cells through dielectrophoresis-based cell stretching and actin cytoskeleton modeling.
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DOI：
10.1186/s12938-017-0329-8
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发表时间：
2017-04-04
影响因子：
3.9
通讯作者：
Sun D
中科院分区：
文献类型：
--
作者：
Bai G;Li Y;Chu HK;Wang K;Tan Q;Xiong J;Sun D
细胞骨架是维持细胞刚性的高度动态网络，细胞的力学性质与细胞的许多功能密切相关。本文提出了一种新的方法来探测和表征细胞的力学性能，通过介电电泳（DEP）为基础的细胞拉伸操纵和肌动蛋白细胞骨架建模。以白血病NB 4细胞为细胞系，观察阿霉素（DOX）化疗后其生物学特性的变化。集成DEP的微流控芯片是一种低成本、高效率的研究细胞变形性的工具。首先通过计算机模拟评估细胞拉伸中使用的DEP力，并将结果与建模方程和光学拉伸（OT）实验的结果进行比较。结构参数，然后提取拟合的实验数据到肌动蛋白细胞骨架模型，和基本的力学性能的细胞随后进行了表征。在不同的电压输入下产生的DEP力进行了计算，从不同的方法的结果表明，很好的近似力估计。DEP和OT拉伸实验都证实了DOX处理的NB4细胞比未处理的细胞更硬。从模型中提取的结构参数和共聚焦图像表明DOX处理后肌动蛋白网络发生了显着变化。建议DEP方法结合肌动蛋白细胞骨架建模是一个简单的工程工具来表征细胞的力学性能。
Cytoskeleton is a highly dynamic network that helps to maintain the rigidity of a cell, and the mechanical properties of a cell are closely related to many cellular functions. This paper presents a new method to probe and characterize cell mechanical properties through dielectrophoresis (DEP)-based cell stretching manipulation and actin cytoskeleton modeling. Leukemia NB4 cells were used as cell line, and changes in their biological properties were examined after chemotherapy treatment with doxorubicin (DOX). DEP-integrated microfluidic chip was utilized as a low-cost and efficient tool to study the deformability of cells. DEP forces used in cell stretching were first evaluated through computer simulation, and the results were compared with modeling equations and with the results of optical stretching (OT) experiments. Structural parameters were then extracted by fitting the experimental data into the actin cytoskeleton model, and the underlying mechanical properties of the cells were subsequently characterized. The DEP forces generated under different voltage inputs were calculated and the results from different approaches demonstrate good approximations to the force estimation. Both DEP and OT stretching experiments confirmed that DOX-treated NB4 cells were stiffer than the untreated cells. The structural parameters extracted from the model and the confocal images indicated significant change in actin network after DOX treatment. The proposed DEP method combined with actin cytoskeleton modeling is a simple engineering tool to characterize the mechanical properties of cells.