Double-stranded RNA interference in the spider Cupiennius salei:: the role of Distal-less is evolutionarily conserved in arthropod appendage formation
Double-stranded RNA interference in the spider Cupiennius salei:: the role of Distal-less is evolutionarily conserved in arthropod appendage formation
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DOI：
10.1007/s004270000121
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发表时间：
2001-02-01
影响因子：
2.4
通讯作者：
Damen, WGM
中科院分区：
文献类型：
--
作者：
Schoppmeier, M;Damen, WGM
螯肢类是节肢动物中的一个基本类群，是研究节肢动物进化过程的一个很好的系统。为了使螯合物的功能研究，我们开发了一个双链RNA干扰（RNAi）协议的蜘蛛，同时研究的Distal少基因的功能。我们从萨氏杯盘蛛（Cupiennius salei）中分离了无远端基因。Cs-Dll基因表达首先见于前体节细胞，然后才长出附属物。附件形成后，Cs-Dll在前体附件的远端部分表达，此外，在阴唇、两对后肢（腹部）肢芽、头部区域和蜘蛛胚胎的最后端也表达。在Dll被RNAi沉默的胚胎中，前体附属物的远端部分缺失，唇完全缺失。因此，Dll在唇形成中也起着至关重要的作用。然而，在RNAi胚胎中完全缺乏唇可能表明唇的性质与节段附属物不同。我们的数据表明，在节肢动物中的附件中的Dll的表达是保守的，而且，Dll的作用是在节肢动物中的节段性附件的形成在进化上保守。
Chelicerates represent a basal arthropod group, which makes them an excellent system for the study of evolutionary processes in arthropods. To enable functional studies in chelicerates, we developed a double-stranded RNA-interference (RNAi) protocol for spiders while studying the function of the Distal-less gene. We isolated the Distal-less gene from the spider Cupiennius salei. Cs-Dll gene expression is first seen in cells of the prosomal segments before the outgrowth of the appendages. After the appendages have formed, Cs-Dll is expressed in the distal portion of the prosomal appendages, and in addition, in the labrum, in two pairs of opisthosmal (abdominal) limb buds, in the head region, and at the posterior-most end of the spider embryo. In embryos, in which Dll was silenced by RNAi, the distal part of the prosomal appendages was missing and the labrum was completely absent. Thus, Dll also plays a crucial role in labrum formation. However, the complete lack of labrum in RNAi embryos may point to a different nature of the labrum from the segmental appendages. Our data show that the expression of Dll in the appendages is conserved among arthropods, and furthermore that the role of Dll is evolutionarily conserved in the formation of segmental appendages in arthropods.