Expression profiles of 4-nonylphenol-exposed medaka (Oryzias latipes) analyzed with a 3.4 K microarray.
Expression profiles of 4-nonylphenol-exposed medaka (Oryzias latipes) analyzed with a 3.4 K microarray.
复制标题
使用 3.4 K 微阵列分析暴露于 4-壬基酚的青鳉 (Oryzias latipes) 的表达谱。
DOI：
10.1016/j.marenvres.2006.04.051
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发表时间：
2006
影响因子：
3.3
通讯作者：
Jae
中科院分区：
文献类型：
--
作者：
Il;Youngmi Lee;Chulwoo Lee;Hyun;S. Oda;Yong;H. Mitani;Jae
利用由3.4万个medaka特异性cDNA探针组成的芯片研究了medaka肝脏中基因表达对4-壬基酚（4-NP）的响应变化。在暴露于两种不同浓度（20和100μg/L）的4-NP 24小时后，我们发现44个不同的基因上调或下调，并有统计学意义的变化。消化酶基因下调，而卵黄蛋白原、绒毛膜原和新型生物标志物基因上调。此外，在100μg/L 4-NP作用下，分别于0、24、48和96h，分析基因表达随时间的变化。75个基因的表达发生了改变，这些基因被分为四个不同的簇。用12个代表性基因的半定量PCR验证了结果的有效性，证实了微阵列数据。因此，我们认为该芯片可用于生成medaka基因表达谱，用于medaka生态毒理学基因组学研究。
Changes in gene expression in liver of medaka in response to 4-nonylphenol (4-NP) were investigated with a microarray consisting of 3.4K medaka-specific cDNA probes. Upon exposure to 4-NP at two different concentrations (20 and 100μg/L) for 24h, we found 44 different genes that were up- or downregulated with statistically significant changes. Digestive enzyme genes were downregulated but vitellogenin, choriogenin and novel biomarker genes were upregulated. Additionally an analysis of the changes of gene expression with respect to time was conducted at 100μg/L of 4-NP with samples at 0, 24, 48 and 96h. The expression of 75 genes was altered and these were categorized into four different clusters. The validity of the results was checked by semi-quantitative PCR with 12 representative genes and this confirmed the microarray data. Therefore, we suggest that this medaka 3.4K chip would be useful in generating gene expression profiles for studies of ecotoxicogenomics in medaka.