Threshold and spectral sensitivity of vision in medaka Oryzias latipes determined by a novel template wave matching method
Threshold and spectral sensitivity of vision in medaka Oryzias latipes determined by a novel template wave matching method
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DOI：
10.1016/j.cbpa.2020.110808
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发表时间：
2021-01-01
影响因子：
2.3
通讯作者：
Kotani, Tomonari
中科院分区：
文献类型：
--
作者：
Hayasaka, Oki;Anraku, Kazuhiko;Kotani, Tomonari
本文提出了一种新的分析方法，用于确定视网膜电图（ERG）中的反应阈值，其中波显示了双相慢直流电位移位。该方法使用记录波的最高强度刺激在每个波长测试作为模板波f（t），并将其与其他记录波下获得的较低强度g（t）。我们的测试记录青鳉latipes之间的模板和比较的波形是类似的，虽然有差异的幅度和时间滞后（tau，峰值时间差）发生的结果，在刺激强度的差异。采用交叉相关分析。基于在每次比较中获得的互相关函数C-fg（tau），tau被确定为互相关系数R-fg（tau）显示最大值的时滞。当采用条件被设置为满足R（tau）>= 0.7和tau时，基于实验者的目视检查和这种新方法确定的阈值一致
We propose a new analytical method for determining the response threshold in electroretinogram (ERG) in which the wave shows a biphasic slow dc-potential shift. This method uses the recorded wave to the highest intensity stimuli in each wavelength tested as a template wave f(t), and it was compared with other recorded waves obtained under lower intensities g(t). Our test recordings in medaka Oryzias latipes were analogous between the template and the compared waveforms, although there were differences in amplitude and time lag (tau, peak time difference) which occurred as a result of the difference in stimulus intensity. Cross-correlation analysis was applied. Based on the obtained cross-correlation function C-fg(tau) in each comparison, tau was determined as the time lag at which the cross-correlation coefficient R-fg(tau) showed the maximum value. Determined thresholds that were based on both the experimenter's visual inspection and this new method agreed well when the adoption condition was set to satisfy R(tau) >= 0.7 and tau