Focused ultrasound-mediated non-invasive brain stimulation: examination of sonication parameters.
Focused ultrasound-mediated non-invasive brain stimulation: examination of sonication parameters.
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DOI：
10.1016/j.brs.2014.06.011
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发表时间：
2014-09
影响因子：
7.7
通讯作者：
Yoo SS
中科院分区：
文献类型：
--
作者：
Kim H;Chiu A;Lee SD;Fischer K;Yoo SS
经颅聚焦超声（FUS）已成为一种新的脑刺激模式。成功脑刺激的超声参数范围需要进一步研究。本研究的目的是检查FUS超声处理参数的范围，这些参数在成功刺激Sprague-Dawley大鼠运动脑区的同时使声强度/能量沉积最小化。我们transcranially管理FUS到大鼠大脑的somatomotor区，并测量声强度，引起兴奋性的影响，相对于不同的脉冲参数（音脉冲持续时间，脉冲重复频率，占空比，和超声处理持续时间）在350和650 kHz的基本频率。我们观察到，在有限的超声参数范围内，即1-5 ms的短纯音持续时间，50%的占空比和300 ms的超声持续时间，在350 kHz的基频下，在最小阈值声强度（空间峰值脉冲平均强度为4.9-5.6 W/cm 2;空间峰值时间平均强度为2.5-2.8 W/cm 2）下引起运动反应。我们还发现，脉冲声处理引起的运动反应，在较低的声强度比其等效的连续声处理。我们的研究结果表明，脉冲应用FUS选择性地刺激特定的大脑区域的利益，在一个声学强度是兼容的监管安全限制的生物组织，从而允许在神经治疗的潜在应用。
Transcranial focused ultrasound (FUS) has emerged as a new brain stimulation modality. The range of sonication parameters for successful brain stimulation warrants further investigation. The objective of this study was to examine the range of FUS sonication parameters that minimize the acoustic intensity/energy deposition while successfully stimulating the motor brain area in Sprague-Dawley rats. We transcranially administered FUS to the somatomotor area of the rat brain and measured the acoustic intensity that caused excitatory effects with respect to different pulsing parameters (tone-burst duration, pulse-repetition frequency, duty cycle, and sonication duration) at 350 and 650 kHz of fundamental frequency. We observed that motor responses were elicited at minimum threshold acoustic intensities (4.9–5.6 W/cm2 in spatial-peak pulse-average intensity; 2.5–2.8 W/cm2 in spatial-peak temporal-average intensity) in a limited range of sonication parameters, i.e. 1–5 ms of tone-burst duration, 50% of duty cycle, and 300 ms of sonication duration, at 350 kHz fundamental frequency. We also found that the pulsed sonication elicited motor responses at lower acoustic intensities than its equivalent continuous sonication. Our results suggest that the pulsed application of FUS selectively stimulates specific brain areas-of-interest at an acoustic intensity that is compatible with regulatory safety limits on biological tissue, thus allowing for potential applications in neurotherapeutics.