A SERS protocol as a potential tool to access 6-mercaptopurine release accelerated by glutathione-S-transferase.
A SERS protocol as a potential tool to access 6-mercaptopurine release accelerated by glutathione-S-transferase.
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DOI：
10.1039/c5an01588j
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发表时间：
2015-10
期刊：
影响因子：
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通讯作者：
Ying Wang;Jie Sun;Qingran Yang;Wenbo Lu;Yan Li;Jian Dong;Weiping Qian
中科院分区：
文献类型：
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作者：
Ying Wang;Jie Sun;Qingran Yang;Wenbo Lu;Yan Li;Jian Dong;Weiping Qian
GST是一种重要的药物代谢酶，其监测方法的建立将极大地促进相关生物学领域的研究。在这项工作中，我们发展了一种表面增强拉曼光谱技术来监测6-巯基嘌呤(6-MP)及其谷胱甘肽-S转移酶(GST)加速的谷胱甘肽(GSH)在金纳米花(GNFS)表面引发的释放行为，将其作为良好的SERS底物。SERS信号被用作6-MP在金表面的吸收或释放的指示剂。我们发现GST可以加速GSH触发的6-MP从金表面的释放行为。我们推测GST催化亲核GSH与亲电物质6-MP竞争结合。实验结果表明，该SERS方法可以作为一种有效的抗癌药物释放监测工具。
The developed method for monitoring GST, an important drug metabolic enzyme, could greatly facilitate researches on relative biological fields. In this work, we have developed a SERS technique to monitor the absorbance behaviour of 6-mercaptopurine (6-MP) and its glutathione-S-transferase (GST)-accelerated glutathione (GSH)-triggered release behaviour on the surface of gold nanoflowers (GNFs), using the GNFs as excellent SERS substrates. The SERS signal was used as an indicator of absorbance or release of 6-MP on the gold surface. We found that GST can accelerate GSH-triggered release behaviour of 6-MP from the gold surface. We speculated that GST catalyzes nucleophilic GSH to competitively bind with the electrophilic substance 6-MP. Experimental results have proved that the presented SERS protocol can be utilized as an effective tool for accessing the release of anticancer drugs.