Functional characterization of the corazonin-encoding gene in phase polyphenism of the migratory locust, Locusta migratoria (Orthoptera: Acrididae)
Functional characterization of the corazonin-encoding gene in phase polyphenism of the migratory locust, Locusta migratoria (Orthoptera: Acrididae)
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DOI：
10.1007/s13355-015-0391-2
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发表时间：
2016-05
影响因子：
1.3
通讯作者：
R. Sugahara;S. Tanaka;A. Jouraku;T. Shiotsuki
中科院分区：
文献类型：
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作者：
R. Sugahara;S. Tanaka;A. Jouraku;T. Shiotsuki
蝗虫表现出阶段多型性，其中发生在响应拥挤条件下的独居（隔离饲养）和群居（群体饲养）表型之间的相变。从独居性到群居性的阶段转变伴随着体色的变暗和经典的形态测量比率如F/C（F后股骨长; C最大头宽）的变化。这些变化发生在没有拥挤的情况下，如果独居蝗虫注射神经肽，corazonin（CRZ）。本研究探讨了CRZ基因敲除对飞蝗Locusta migratoria（L.）（直翅目：蝗科）。注射Crz的dsRNA显著降低了CRZmRNA水平，并降低了前背板上的变暗强度。CRZ在群居性果蝇中表达的沉默使F/C向成虫阶段独居型的典型值移动。然而，CRZ的表达谱在群居和独居的鸟类之间是相似的。因此，我们得出结论，Crz是负责的阶段依赖性的变化，变暗和形态比例，但是，这些变化并不控制通过differentialCRZ表达在转录水平。
Locusts exhibit phase polyphenism in which phase transition between the solitarious (isolation-reared) and gregarious (crowd-reared) phenotypes occurs in response to crowding conditions. Phase transformation from solitarious to gregarious nymphs is accompanied by darkening of the body color and changes in classical morphometric ratios such asF/C(Fhind femur length;Cmaximum head width). These changes occur in the absence of crowding if solitarious locusts are injected with the neuropeptide, corazonin (Crz). This study investigated the effects of the knockdown of theCRZgene on body color and morphometric characteristics in the migratory locustLocusta migratoria(L.) (Orthoptera: Acrididae). An injection of dsRNA for Crz significantly reducedCRZmRNA levels and reduced the intensity of darkening on the pronotum in nymphs. The silencing ofCRZexpression in gregarious nymphs shifted theF/Ctoward a value typical of the solitarious form in the adult stage. However, the expression profiles ofCRZwere similar between the gregarious and solitarious nymphs. Therefore, we conclude that Crz is responsible for phase-dependent changes in darkening and the morphometric ratio; however, these changes are not controlled through differentialCRZexpression at the transcriptional level.