Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes.
Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes.
复制标题
DOI：
10.1093/aob/mcx149
复制
发表时间：
2018-02-12
期刊：
影响因子：
4.2
通讯作者：
King IP
中科院分区：
文献类型：
--
作者：
King J;Grewal S;Yang CY;Hubbart Edwards S;Scholefield D;Ashling S;Harper JA;Allen AM;Edwards KJ;Burridge AJ;King IP
面包小麦(Triticum Aestivum)在进化和驯化过程中经历了严重的遗传瓶颈。因此，产生和利用新的遗传变异来源是至关重要的。本研究旨在从斯卑尔托山羊草中产生全基因组的导入片段。这项研究产生的导入基因将用于表型分析。斯氏山羊草(Aegilopspeltoides)作为普通小麦‘Paragon’的父本杂交而成。然后，这些种间杂交种回交到帕拉贡。用Affymetrix Axiom芯片和基因组原位杂交(GISH)检测和鉴定导入基因。F_1代配子的重组达到了产生Ae的遗传连锁图谱的水平。斯佩尔图里德。利用该方法对294个小麦/Ae进行了鉴定。斯佩尔托德引种。7个连锁群的引种。发现了包括大节和小节在内的石器。对比分析表明，Ae与Ae的宏观共时关系总体上是保守的。Speltoids和T.aestivum，但那是Ae.Speltoides不包含小麦中存在的4A/5A/7B易位。据报道，斯氏山羊草携带杀配子基因，即确保它们通过配子传递给下一代的基因。传输速率的七个Ae。导入小麦的SPELTOIDES连锁群多种多样。连锁群2的100%传递率表明该染色体上存在杀配子基因。小麦和Ae染色体之间发生了高度的重组。Speltoides，导致产生大量具有在育种计划中利用潜力的导入。由于有杀配子的基因，所有的种质都会含有Ae的片段。Speltoides连锁群2S，以及来自任何其他连锁群的导入。
Bread wheat (Triticum aestivum) has been through a severe genetic bottleneck as a result of its evolution and domestication. It is therefore essential that new sources of genetic variation are generated and utilized. This study aimed to generate genome-wide introgressed segments from Aegilops speltoides. Introgressions generated from this research will be made available for phenotypic analysis. Aegilops speltoides was crossed as the male parent to T. aestivum ‘Paragon’. The interspecific hybrids were then backcrossed to Paragon. Introgressions were detected and characterized using the Affymetrix Axiom Array and genomic in situ hybridization (GISH). Recombination in the gametes of the F1 hybrids was at a level where it was possible to generate a genetic linkage map of Ae. speltoides. This was used to identify 294 wheat/Ae. speltoides introgressions. Introgressions from all seven linkage groups of Ae. speltoides were found, including both large and small segments. Comparative analysis showed that overall macro-synteny is conserved between Ae. speltoides and T. aestivum, but that Ae. speltoides does not contain the 4A/5A/7B translocations present in wheat. Aegilops speltoides has been reported to carry gametocidal genes, i.e. genes that ensure their transmission through the gametes to the next generation. Transmission rates of the seven Ae. speltoides linkage groups introgressed into wheat varied. A 100 % transmission rate of linkage group 2 demonstrates the presence of the gametocidal genes on this chromosome. A high level of recombination occurs between the chromosomes of wheat and Ae. speltoides, leading to the generation of large numbers of introgressions with the potential for exploitation in breeding programmes. Due to the gametocidal genes, all germplasm developed will always contain a segment from Ae. speltoides linkage group 2S, in addition to an introgression from any other linkage group.
登录
查看更多内容
影响因子：
1.6
作者：
Friebe, B;Zhang, P;Gill, BS
通讯作者：
Gill, BS
影响因子：
1.5
作者：
Haldane, JBS
通讯作者：
Haldane, JBS
影响因子：
3.1
作者：
KING, IP;KOEBNER, RMD;LAW, CN
通讯作者：
LAW, CN
影响因子：
5.4
作者：
KING, IP;MILLER, TE;KOEBNER, RMD
通讯作者：
KOEBNER, RMD
影响因子：
5.4
作者：
Maestra, B;Naranjo, T
通讯作者：
Naranjo, T