Factors affecting human supragingival biofilm composition. II. Tooth position
Factors affecting human supragingival biofilm composition. II. Tooth position
复制标题
DOI：
10.1111/j.1600-0765.2008.01155.x
复制
发表时间：
2009-08-01
影响因子：
3.5
通讯作者：
Socransky, S. S.
中科院分区：
文献类型：
--
作者：
Haffajee, A. D.;Teles, R. P.;Socransky, S. S.
背景与目的：影响龈上生物膜微生物组成的因素尚不清楚。本研究旨在验证牙齿位置对龈上菌斑微生物组成的影响，其影响超出了由总DNA探针计数反映的菌斑质量。材料和方法：187名受试者（n = 4745份样本）从每颗牙齿的中颊面采集龈上菌斑样本。采用棋盘格DNA-DNA杂交技术对所有样品进行了40种细菌的含量分析。采用Kruskal-Wallis检验，分别测定下颌和上颌骨中磨牙、二尖牙、门牙/犬齿6个牙型类别中平均物种数和比例差异的显著性。采用逐步多元线性回归分析物种比例与DNA探针总数、牙位、牙周和吸烟状况、年龄和性别之间的关系。结果：所有种在牙型和6个牙类之间存在显著差异。臼齿和下门牙的牙菌斑水平较高。在牙齿类型之间观察到的一些差异可以部分地用牙菌斑的水平来解释。牙菌斑质量高的牙菌斑中，牙龈嗜糖细胞噬菌、耐氏放线菌2基因种、直弯曲菌和弯曲菌含量高。然而，某些物种，如细小微孔球菌和血链球菌，尽管在牙菌斑质量上相似，但在不同的牙齿位置存在显著差异。在调整了总DNA探针计数和受试者水平因素后，20种被试物种与牙齿位置表现出显著的相关性。结论：尽管牙菌斑质量与龈上生物膜中许多物种的比例差异有关，但在单因素和多因素分析中，牙齿位置也与物种比例密切相关。
Background and Objective:Little is known regarding the factors that affect the microbial composition of supragingival biofilms. This study was designed to test the hypothesis that tooth location affects the microbial composition of supragingival plaque beyond the effect due to plaque mass as reflected by total DNA probe count.Material and Methods:Supragingival plaque samples were taken from the mesiobuccal aspect of each tooth in 187 subjects (n = 4745 samples). All samples were individually analyzed for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. Significance of differences in mean species counts and proportions were determined among tooth surfaces and six tooth type categories: molars, bicuspids, incisors/canines in the mandible and maxilla separately using the Kruskal-Wallis test. Stepwise multiple linear regression was employed to examine the relationship between species proportions and total DNA probe count, tooth location, periodontal and smoking status, age and sex.Results:All species differed significantly among tooth types and among the six tooth categories. Higher plaque levels were seen on molars and lower incisors. Some differences observed between tooth types could be partly explained by the level of plaque. Teeth with high plaque mass exhibited high levels of Capnocytophaga gingivalis, Actinomyces naeslundii genospecies 2, Campylobacter rectus and Campylobacter showae. However, certain species, such as Veillonella parvula and Streptococcus sanguinis, differed significantly at different tooth locations despite similarities in plaque mass. Twenty of the test species exhibited a significant association with tooth location after adjusting for total DNA probe count and subject level factors.Conclusion:While plaque mass was associated with differences in proportions of many species in supragingival biofilms, tooth location also was strongly associated with species proportions in both univariate and multivariate analyses.