Bacterial and archaeal communities in the acid pit lake sediments of a chalcopyrite mine
Bacterial and archaeal communities in the acid pit lake sediments of a chalcopyrite mine
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DOI：
10.1007/s00792-013-0576-y
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发表时间：
2013-11-01
期刊：
影响因子：
2.9
通讯作者：
Lambais, M. R.
中科院分区：
文献类型：
--
作者：
Lucheta, A. R.;Otero, X. L.;Lambais, M. R.
采用16S rRNA基因PCR-DGGE和克隆文库测序的方法，对西班牙图罗某黄铜矿酸性坑湖3种不同沉积环境(BH1A型、BH2A型和BH3A型)的细菌和古菌群落结构及多样性进行了研究。细菌和古生菌扩增产物的DGGE分析表明，沉积物中含有不同的群落。将细菌16S rRNA基因序列聚为42个可操作分类单元(OTU)，将其归入酸杆菌、放线菌、蓝藻、植物霉菌、变形杆菌、绿藻和未培养细菌。OTU-2分别占底泥BH1A、BH2A和BH3A全部序列的37%、42%和37%，与未培养的Chloroflei有亲缘关系。其余的OTU与异养菌有亲缘关系，包括铁丝菌属和酸杆菌属的代表。古细菌16S rRNA基因序列聚为54个OTU。BH1a沉积物中的大部分序列归属于Eurya chaeota，而BH2A沉积物中的大部分序列归属于Cren考古纲。BH3A沉积物中的大部分序列被归入未分类的古生代，并与未培养和未分类的环境克隆显示出相似性。在本研究中没有发现与酸性矿山废水有关的酸性硫杆菌和钩端螺旋菌的相关序列。
Bacterial and archaeal community structures and diversity of three different sedimentary environments (BH1A, BH2A and BH3A) in the acid pit lake of a chalcopyrite mine at Touro (Spain) were determined by 16S rRNA gene PCR-DGGE and sequencing of clone libraries. DGGE of bacterial and archaeal amplicons showed that the sediments harbor different communities. Bacterial 16S rRNA gene sequences were assigned to Acidobacteria, Actinobacteria, Cyanobacteria, Planctomycetes, Proteobacteria, Chloroflexi and uncultured bacteria, after clustering into 42 operational taxonomic units (OTUs). OTU 2 represented approximately 37, 42 and 37 % of all sequences from sediments BH1A, BH2A and BH3A, respectively, and was phylogenetically related to uncultured Chloroflexi. Remaining OTUs were phylogenetically related to heterotrophic bacteria, including representatives of Ferrithrix and Acidobacterium genera. Archaeal 16S rRNA gene sequences were clustered into 54 OTUs. Most of the sequences from the BH1A sediment were assigned to Euryarchaeota, whereas those from BH2A sediment were assigned to Crenarchaeota. The majority of the sequences from BH3A sediment were assigned to unclassified Archaea, and showed similarities to uncultured and unclassified environmental clones. No sequences related to Acidithiobacillus and Leptospirillum, commonly associated with acid mine drainage, were detected in this study.