3D-printable supramolecular hydrogels with shear-thinning property: fabricating strength tunable bioink via dual crosslinking
3D-printable supramolecular hydrogels with shear-thinning property: fabricating strength tunable bioink via dual crosslinking
复制标题
具有剪切稀化特性的3D打印超分子水凝胶：通过双交联制造强度可调的生物墨水
DOI：
10.1016/j.bioactmat.2020.06.001
复制
发表时间：
2020-12-01
影响因子：
18.9
通讯作者：
Fu, Xiaobing
中科院分区：
文献类型：
--
作者：
Hu, Tian;Cui, Xiaoliang;Fu, Xiaobing
3-三维（3D）生物打印技术为人工组织和器官的制造提供了有前途的策略。作为生物打印过程的基本要素，在不牺牲生物相容性的前提下制备具有理想机械性能的生物墨水是一个巨大的挑战。本文以聚乙二醇（PEG）接枝壳聚糖、α-环糊精（α-CD）和明胶为原料，制备了一种超分子水凝胶生物墨水。它通过由α-CD和PEG侧链之间的主客体相互作用形成的假聚轮烷样侧链的聚集而具有一级交联结构。表观粘度的测量显示了这种生物墨水的剪切-光泽特性，这可能是由于物理交联的可逆性。此外，使用不同浓度的β-甘油磷酸盐作为次级交联剂，打印的构建体显示出不同的杨氏模量（p < 0.001）。它们还可以在细胞培养条件下保持杨氏模量至少21天（p < 0.05）。通过将每种组分与成纤维细胞共培养，CCK-8测定表明细胞活力高于80%。生物打印和培养后，免疫荧光染色定量显示Ki-67、Paxillin和N-cadherin的表达在第14天高于第3天（p < 0.05）。油红0和尼氏体特异性染色反映强度可调的生物墨水可能对间充质干细胞的细胞命运有影响（p < 0.05）。本研究为先进的生物墨水在复杂组织器官重建中的应用提供了新的思路。
3-dimensional (3D) bioprinting technology provides promising strategy in the fabrication of artificial tissues and organs. As the fundamental element in bioprinting process, preparation of bioink with ideal mechanical properties without sacrifice of biocompatibility is a great challenge. In this study, a supramolecular hydrogel-based bioink is prepared by polyethylene glycol (PEG) grafted chitosan, alpha-cyclodextrin (alpha-CD) and gelatin. It has a primary crosslinking structure through the aggregation of the pseudo-polyrotaxane-like side chains, which are formed from the host-guest interactions between alpha-CD and PEG side chain. Apparent viscosity measurement shows the shear-shinning property of this bioink, which might be due to the reversibility of the physical crosslinking. Moreover, with beta-glycerophosphate at different concentrations as the secondary crosslinking agent, the printed constructs demonstrate different Young's modulus (p < 0.001). They could also maintain the Young's modulus in cell culture condition for at least 21 days (p < 0.05). By co-culturing each component with fibroblasts, CCK-8 assay demonstrate cellular viability is higher than 80%. After bioprinting and culturing, immunofluorescence staining with quantification indicate the expression of Ki-67, Paxillin, and N-cadherin is higher in day 14 than those in day 3 (p < 0.05). Oil red 0 and Nissl body specific staining reflect strength tunable bioink may have impact on the cell fate of mesenchymal stem cells (p < 0.05). This work might provide new idea for advanced bioink in the application of re-establishing complicated tissues and organs.