SELF-TRAPPING AND ANOMALOUS DISPERSION OF DNA IN ELECTROPHORESIS
SELF-TRAPPING AND ANOMALOUS DISPERSION OF DNA IN ELECTROPHORESIS
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DOI：
10.1103/physrevlett.58.2428
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发表时间：
1987-06-08
影响因子：
8.6
通讯作者：
LALANDE, M
中科院分区：
文献类型：
--
作者：
NOOLANDI, J;ROUSSEAU, J;LALANDE, M
我们提出了一个凝胶电泳的高场偏压爬行模型。据预测，在它们的迁移过程中，DNA片段可以被困在近零速度，looplike紧凑的构象长时间。因此，DNA的电泳迁移率被发现是片段大小的非单调函数。我们目前的实验结果表明，这种独特的和意想不到的后果，由于DNA自陷在紧凑的状态的异常色散。
We present a high-field biased reptation model of gel electrophoresis. It is predicted that during their migration, DNA fragments can get trapped for long periods of time in near-zero–velocity, looplike compact conformations. As a consequence, the electrophoretic mobility of DNA is found to be a nonmonotonic function of the fragment size. We present experimental results showing this unique and unexpected consequence of anomalous dispersion due to DNA self-trapping in compact states.