Effects of intermittent or continuous gravitational stresses on cell-matrix adhesion: Quantitative analysis of focal contacts in osteoblastic ROS 17/2.8 cells
Effects of intermittent or continuous gravitational stresses on cell-matrix adhesion: Quantitative analysis of focal contacts in osteoblastic ROS 17/2.8 cells
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DOI：
10.1006/excr.1997.3703
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发表时间：
1997-10-10
影响因子：
3.7
通讯作者：
Vico, L
中科院分区：
文献类型：
--
作者：
Guignandon, A;Usson, Y;Vico, L
细胞形态和细胞代谢之间的关系以及机械负荷在骨重塑中的作用是众所周知的。机械刺激会引起成骨细胞形状的变化，这可能是由焦点接触的重组介导的。我们研究了抛物线飞行期间发生的重力 (Gz) 变化对 ROS 17/2.8 骨肉瘤细胞进行 15 或 30 次抛物线飞行的成骨细胞粘着斑的影响。飞行引起的显着形状变化包括与焦点接触斑块重组相关的细胞面积减少。由离心（2 Gz）或旋转（Gz 随机化）引起的相同持续时间的连续机械应力对细胞粘着斑没有重大影响。用诺考达唑处理的 ROS 17/2.8 G2/M 同步抑制了飞行引起的粘附参数下降。我们得出的结论是，ROS 17/2.8 细胞对 Gz 开关敏感，并且它们的适应至少依赖于微管功能。 (C) 1997 年学术出版社。
The relationship between cell morphology and cell metabolism and the role of mechanical load in bone remodeling is well known. Mechanical stimulation induces changes in the shape of osteoblasts, probably mediated by reorganization of focal contacts. We studied the influence of gravity (Gz) variations occurring during parabolic flight on osteoblast focal adhesion of ROS 17/2.8 osteosarcoma cells subjected to 15 or 30 parabolic flights. Significant flight-induced shape changes consisted of decreased cell area associated with focal contact plaque reorganization. Identical durations of continuous mechanical stress induced by centrifugation (2 Gz) or clinorotation (Gz randomization) had no major effect on cell focal adhesion. ROS 17/2.8 G2/M synchronization by treatment with nocodazole inhibited the flight-induced decrease in adhesion parameters. We concluded that ROS 17/2.8 cells are sensitive to Gz switches and that their adaptation is at least dependent on microtubule function. (C) 1997 Academic Press.