The Hypolipidemic Effect of Active Components in the Decoction of Alisma Orientale and their Chemical Structures Characterized by LC-QTOF-MS/MS
The Hypolipidemic Effect of Active Components in the Decoction of Alisma Orientale and their Chemical Structures Characterized by LC-QTOF-MS/MS
复制标题
泽泻汤剂中活性成分的降血脂作用及其化学结构的LC-QTOF-MS/MS表征
DOI：
10.2174/1573412915666190207151908
复制
发表时间：
2020-01-01
影响因子：
0.6
通讯作者：
Song，Chengwu
中科院分区：
文献类型：
--
作者：
Shi，Qingxin;Zhang，Qiongguang;Song，Chengwu
泽泻的水煎液被用作传统药物，
治疗高脂血症在中国具有悠久的临床历史。本研究进行了详细的
目的：比较两种中药提取物的降血脂作用及化学成分
泽泻分别采用沸水和有机试剂法制备。
高脂血症小鼠通过给予高脂饮食（HFD）诱导，
月小鼠体重、血清总胆固醇（TC）、高密度脂蛋白胆固醇（HDL
超氧化物歧化酶（SOD）、丙二醛（MDA）、天冬氨酸转氨酶（AST）
并测定丙氨酸氨基转移酶（ALT）水平。同时，还研究了其化学成分
采用液相色谱-四极杆飞行时间质谱对提取物进行了表征
（LC-QTOF-MS/MS）。
结果表明，该方具有较好的降血脂作用，
与甲醇提取物相比。此外，通过照片证实了肝脏保护作用
油红脂染色。提取物中的化学成分主要有20种
利用LC-QTOF-MS/MS鉴定或表征了组分，其中，8个寡肽
首次对泽泻水煎液中的生物碱成分进行了定性和定量研究，证明了泽泻水煎液中的生物碱成分。
有理由认为寡肽可能部分有助于降低血脂，
泽泻的功效结构表征程序还提供了合适的
方法分析植物样品中的寡肽类似物。
The decoction of Alisma orientale is used as a traditional medicine for the
treatment of hyperlipidemia in China with a long clinical history. The present study undertook a detailed
investigation to compare the hypolipidemic effect and chemical composition of two extracts of
Alisma orientale prepared by boiling water and organic reagent, respectively.
The hyperlipidemic mice were induced by administration of a High-Fat Diet (HFD) for one
month. The body weight of mice and the serum Cholesterol (TC), high-density lipoprotein cholesterol
(HDL-C), Superoxide Dismutase (SOD), malondialdehyde (MDA), aspartate Aminotransferase (AST)
and alanine aminotransferase (ALT) levels were determined. Meanwhile, the chemical constituents of
the extracts were characterized using liquid chromatography-quadrupole time of flight mass spectrometry
(LC-QTOF-MS/MS).
The better hypolipidemic effect was observed in mice administered with the decoction compared
to that of methanol extract. In addition, the liver protective effects were confirmed by the photographs
stained with oil red lipid stain. As for the chemical constituents in the extracts, twenty major
components were identified or characterized using LC-QTOF-MS/MS. Among them, eight oligopeptides
were characterized for the first time and proved to only exist in the decoction of Alisma orientale.
It is reasonable to assume that the oligopeptides may partially contribute to the hypolipidemic
effect of Alisma orientale. The structural characterization procedures also provide a suitable
method to analyze oligopeptide analogues in plant samples.