REPRESSION BY THE YEAST MEIOTIC INHIBITOR RME1
REPRESSION BY THE YEAST MEIOTIC INHIBITOR RME1
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DOI：
10.1101/gad.7.8.1598
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发表时间：
1993-08-01
影响因子：
10.5
通讯作者：
MITCHELL, AP
中科院分区：
文献类型：
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作者：
COVITZ, PA;MITCHELL, AP
RME 1基因产物是减数分裂的负调控因子，具有三个锌指基序，通过阻止IME 1的转录物积累而起作用，IME 1的产物是减数分裂基因表达所需的。我们已经从IME 1上游2 kb的区域中分离出了一个404 bp的片段，该片段足以用于RME 1依赖性抑制异源启动子。该DNA包含RME 1反应元件（RRE）和另一个称为调节区的区域。调控区是阻遏所必需的，因为含有RRE的DNA单独不阻遏，但能够赋予RME 1依赖的转录激活的报告基因。在凝胶流动性阻滞试验中，RME 1与RRE形成特异性复合物，降低RME 1亲和力的RRE点突变也阻断了阻遏和激活。RME 1-RRE复合物的足迹显示了一个21 bp的保护区，其中包括这些RRE突变的位置。我们的结论是，RME 1结合到这个RRE是需要镇压。因此，RME 1抑制减数分裂的机制是IME 1的直接转录抑制。
The RME1 gene product, a negative regulator of meiosis with three zinc finger motifs, acts by preventing transcript accumulation from IME1, whose product is required for meiotic gene expression. We have isolated a 404-bp segment from a region 2 kb upstream of IME1 that is sufficient for RME1-dependent repression of a heterologous promoter. This DNA contains an RME1-response element (RRE) and another region called the modulation region. The modulation region is required for repression because DNA containing the RRE alone did not repress but was able to confer RME1-dependent transcriptional activation of a reporter gene. In gel mobility retardation assays, RME1 formed a specific complex with the RRE, and RRE point mutations that reduced the affinity for RME1 also blocked repression and activation. Footprinting of the RME1-RRE complex revealed a 21-bp protected region that included the positions of these RRE mutations. We conclude that RME1 binding to this RRE is required for repression. Thus, the mechanism of meiotic inhibition by RME1 is direct transcriptional repression of IME1.