A Splice Variant of Centrosomin Converts Mitochondria to Microtubule-Organizing Centers.
A Splice Variant of Centrosomin Converts Mitochondria to Microtubule-Organizing Centers.
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DOI：
10.1016/j.cub.2017.05.090
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发表时间：
2017-07-10
期刊：
影响因子：
--
通讯作者：
Megraw TL
中科院分区：
文献类型：
--
作者：
Chen JV;Buchwalter RA;Kao LR;Megraw TL
Non-centrosomal Microtubule Organizing Centers (MTOCs) direct microtubule (MT) organization to exert diverse cell-type specific functions. In Drosophila spermatids, the giant mitochondria provide structural platforms for MT reorganization to support elongation of the extremely long sperm. However, the molecular basis for this mitochondrial MTOC and other non-centrosomal MTOCs have not been discerned. Here we report that Drosophila centrosomin (cnn) expresses two major protein variants: the centrosomal form (CnnC) and a non-centrosomal form in testes (CnnT). CnnC is established as essential for functional centrosomes, the major MTOCs in animal cells. We show that CnnT is expressed exclusively in testes by alternative splicing, and localizes to giant mitochondria in spermatids. In cell culture, CnnT targets to the mitochondrial surface, recruits the MT nucleator γ-TuRC, and is sufficient to convert mitochondria to MTOCs independent of core pericentriolar proteins that regulate MT assembly at centrosomes. We mapped two separate domains in CnnT. One that is necessary and sufficient to target it to mitochondria, and another that is necessary and sufficient to recruit γ-TuRCs and nucleate MTs. In elongating spermatids, CnnT forms speckles on the giant mitochondria that are required to recruit γ-TuRCs to organize MTs and support spermiogenesis. This molecular characterization of the mitochondrial MTOC defines a minimal molecular requirement for MTOC generation, and implicates the potent role of Cnn (or its related) proteins in the direct regulation of MT assembly and organization of non-centrosomal MTOCs.
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影响因子：
3.3
作者：
Fong, Ka-Wing;Choi, Yuk-Kwan;Qi, Robert Z.
通讯作者：
Qi, Robert Z.
影响因子：
7.8
作者：
Haren, Laurence;Remy, Marie-Helene;Bazin, Ingrid;Callebaut, Isabelle;Wright, Michel;Merdes, Andreas
通讯作者：
Merdes, Andreas
DOI：
10.1083/jcb.141.1.163
发表时间：
1998-04-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dictenberg JB;Zimmerman W;Sparks CA;Young A;Vidair C;Zheng Y;Carrington W;Fay FS;Doxsey SJ
通讯作者：
Doxsey SJ
影响因子：
23.9
作者：
Gabriel, Elke;Ramani, Anand;Gopalakrishnan, Jay
通讯作者：
Gopalakrishnan, Jay
影响因子：
7.8
作者：
HOYLE, HD;RAFF, EC
通讯作者：
RAFF, EC