Novel crAssphage marker genes ascertain sewage pollution in a recreational lake receiving urban stormwater runoff
Novel crAssphage marker genes ascertain sewage pollution in a recreational lake receiving urban stormwater runoff
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DOI：
10.1016/j.watres.2018.08.049
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发表时间：
2018-11-15
期刊：
影响因子：
12.8
通讯作者：
Power, Kaye
中科院分区：
文献类型：
--
作者：
Ahmed, Warish;Payyappat, Sudhi;Power, Kaye
近年来，在开发用于环境沃茨中粪便污染追踪的新型标记基因方面已经做出了相当大的努力。噬菌体是最近发现的DNA噬菌体，在人类粪便和未经处理的污水中高度丰富。在这项研究中，我们评估了新设计的crAsphalage qPCR测定的宿主敏感性和特异性（Stachler等人，2017）CPQ_056和CPQ_064（即，标记基因）。我们还研究了这些标记基因在检测城市休闲湖泊（即，新南威尔士州悉尼的帕拉马塔湖）。未处理污水中CPQ_056和CPQ_064标记基因的平均浓度分别为9.43 +/- 0.14 log（10）GC/L和8.91 +/- 0.17 log（10）GC/L，比微生物来源追踪研究中使用的其他污水相关病毒高2至3个数量级。在来自11个物种的177份动物粪便样品中，CPQ_056和CPQ_064标记基因的宿主特异性值分别为0.95和0.93。在猫粪便和牛废水样品中观察到有限的交叉反应性。在一个接收暴雨径流的城市湖泊中，监测了crAsbrilage标志物的变化。两种标记物的浓度均较高在20/20份样本中（CPQ_056范围为3.40 - 6.04 log（10）GC/L，CPQ_064范围为2.90 - 5.47 log（10）GC/L）（CPQ_056）和18/20（对于CPQ_064）与干燥天气事件相比，在暴雨事件后采集的样本，测量下水道溢流（10个样品中的10个对CPQ_056为qPCR阴性，10个样品中的8个对CPQ_064标记基因为阴性）表明污水污染通过城市雨水径流输送到帕拉马塔湖。研究结果可为集水区污水系统溢流点的污水污染管理提供背景。皇冠版权所有（C）2018由爱思唯尔有限公司发布。保留所有权利。
Considerable efforts have been made in recent years in developing novel marker genes for fecal pollution tracking in environmental waters. CrAssphage are recently discovered DNA bacteriophage that are highly abundant in human feces and untreated sewage. In this study, we evaluated the host-sensitivity and -specificity of the newly designed crAssphage qPCR assays (Stachler et al., 2017) CPQ_056 and CPQ_064 (i.e., marker genes) in fecal samples collected from various human and several animal host groups in Australia. We also investigated the utility of these marker genes to detect sewage pollution in an urban recreational lake (i.e., Lake Parramatta) in Sydney, NSW. The mean concentrations of CPQ_056 and CPQ_064 marker genes in untreated sewage were 9.43 +/- 0.14 log(10) GC/L and 8.91 +/- 0.17 log(10) GC/L, respectively, 2 to 3 orders of magnitude higher than other sewage-associated viruses used in microbial source tracking studies. Among 177 animal fecal samples tested from 11 species, the host-specificity values for CPQ_056 and CPQ_064 marker genes were 0.95 and 0.93, respectively. Limited cross reactivity was observed with cat fecal and cattle wastewater samples. Abundance of crAssphage markers were monitored in an urban lake that receives stormwater runoff. The concentrations of both markers were higher (CPQ_056 ranging from 3.40 to 6.04 log(10) GC/L and CPQ_064 ranging from 2.90 to 5.47 log(10) GC/L) in 20 of 20 (for CPQ_056) and 18 of 20 (for CPQ_064) samples collected after storm events with gauged sewer overflows compared to dry weather event (10 of 10 samples were qPCR negative for the CPQ_056 and 8 of 10 were negative for the CPQ_064 marker genes) suggesting sewage pollution was transported by urban stormwater runoff to Lake Parramatta. The results of the study may provide context for management of sewage pollution from gauged overflow points of the sewerage system in the catchment. Crown Copyright (C) 2018 Published by Elsevier Ltd. All rights reserved.