Opiates and cultured neuroblastoma x glioma cells. Effect on cyclic AMP and polyamine levels and on ornithine decarboxylase and protein kinase activities.
Opiates and cultured neuroblastoma x glioma cells. Effect on cyclic AMP and polyamine levels and on ornithine decarboxylase and protein kinase activities.
复制标题
阿片类药物和培养的神经母细胞瘤 x 神经胶质瘤细胞。
DOI：
10.1042/bj2270389
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
U. Bachrach
中科院分区：
文献类型：
--
作者：
D. Benalal;U. Bachrach
在培养的NG 108-15神经母细胞瘤×神经胶质瘤细胞中，阿片类药物降低细胞环AMP和多胺水平。这种下降与细胞急性暴露于药物期间对鸟苷酸脱羧酶和环腺苷酸依赖性蛋白激酶活性的抑制有关。在阿片类药物存在的情况下生长细胞数天导致药物成瘾。在耐受成瘾的细胞，多胺和环AMP水平接近正常值的活动，鸟氨酸脱羧酶和环AMP依赖性蛋白激酶。通过洗涤或加入拮抗剂纳洛酮从“成瘾”细胞中去除阿片剂，导致环AMP和多胺水平增加，以及鸟氨酸脱羧酶和环AMP依赖性蛋白激酶的活性增加。阿片类药物的作用与其生物活性密切相关。不活跃的对映体不影响环AMP或多胺的水平，也没有减少鸟氨酸脱羧酶和环AMP依赖性蛋白激酶的活动。
In cultured NG 108-15 neuroblastoma x glioma cells, opiates decreased cellular cyclic AMP and polyamine levels. This decrease was related to the inhibition of ornithine decarboxylase and cyclic AMP-dependent protein kinase activities during the acute exposure of the cells to the drugs. Growing the cells in the presence of opiates for several days led to drug addiction. In the tolerant-addicted cells, polyamine and cyclic AMP levels were close to normal values as were the activities of ornithine decarboxylase and cyclic AMP-dependent protein kinase. Removal of the opiate from 'addicted' cells, by either washing or by adding the antagonist naloxone, resulted in an increase in cyclic AMP and polyamine levels and the activities of ornithine decarboxylase and cyclic AMP-dependent protein kinase. The effect of opiates was closely related to their biological activities. Inactive enantiomorphs did not affect cyclic AMP or polyamine levels; neither did they decrease ornithine decarboxylase and cyclic AMP-dependent protein kinase activities.