Diversity of genetic lesions characterizes new Arabidopsis flavonoid pigment mutant alleles from T-DNA collections
Diversity of genetic lesions characterizes new Arabidopsis flavonoid pigment mutant alleles from T-DNA collections
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DOI：
10.1016/j.plantsci.2019.110335
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发表时间：
2020-02-01
期刊：
影响因子：
5.2
通讯作者：
Grotewold, Erich
中科院分区：
文献类型：
--
作者：
Jiang, Nan;Lee, Yun Sun;Grotewold, Erich
类黄酮色素提供的视觉表型为现代遗传学提供了宝贵的工具。许多拟南芥透明种皮突变体缺乏特征性的原花色素（PA）种皮色素沉着，往往不能积累花色素苷在营养组织已被确定。这些突变体对我们理解类黄酮的生物合成、调控和运输有重要贡献。在可用的大T-DNA收集线的TT突变体的全面筛选导致16个独立的线缺乏PA和花青素的鉴定，或与种皮色素明显不同于野生型。分离分析和第二个等位基因的特征的索引T-DNA插入破坏的基因表明，所有的线包含至少一个额外的突变负责的TT表型。使用RNA-Seq和全基因组重新测序的组合并通过互补确认，我们在这里显示这些突变对应于ttg 1（两个等位基因），tt 3（两个等位基因），tt 5（两个等位基因），ban（两个等位基因），tt 1（两个等位基因）和tt 8（六个等位基因）的新等位基因，这些等位基因包含额外的T-DNA插入，插入缺失，错义突变和大基因组缺失。几个确定的等位基因提供了有趣的观点类黄酮的生物合成和调节。
The visual phenotypes afforded by flavonoid pigments have provided invaluable tools for modern genetics. Many Arabidopsis transparent testa (tt) mutants lacking the characteristic proanthocyanidin (PA) seed coat pigmentation and often failing to accumulate anthocyanins in vegetative tissues have been characterized. These mutants have significantly contributed to our understanding of flavonoid biosynthesis, regulation, and transport. A comprehensive screening for tt mutants in available large T-DNA collection lines resulted in the identification of 16 independent lines lacking PAs and anthocyanins, or with seed coat pigmentation clearly distinct from wild type. Segregation analyses and the characterization of second alleles in the genes disrupted by the indexed T-DNA insertions demonstrated that all the lines contained at least one additional mutation responsible for the tt phenotypes. Using a combination of RNA-Seq and whole genome re-sequencing and confirmed through complementation, we show here that these mutations correspond to novel alleles of ttg1 (two alleles), tt3 (two alleles), tt5 (two alleles), ban (two alleles), tt1 (two alleles), and tt8 (six alleles), which harbored additional T-DNA insertions, indels, missense mutations, and large genomic deletion. Several of the identified alleles offer interesting perspectives on flavonoid biosynthesis and regulation.