Stability and degradation of indocyanine green in plasma, aqueous solution and whole blood
Stability and degradation of indocyanine green in plasma, aqueous solution and whole blood
复制标题
DOI：
10.1039/c8pp00064f
复制
发表时间：
2018-09-01
影响因子：
3.1
通讯作者：
Nowak, Kai
中科院分区：
文献类型：
--
作者：
Mindt, Sonani;Karampinis, Ioannis;Nowak, Kai
近年来，基于双折射的成像已经发展成为一种重要的技术。特别是吲哚菁绿色（ICG）已侵入诊断和介入医学的大多数领域。考虑到该物质的众多优点，如快速降解和罕见的不良反应，ICG是目前最常用的荧光剂。采用高效液相色谱法（HPLC）测定了ICG及其潜在降解物的吸光度和荧光。在各种温度下，在光暴露或黑暗中评价稳定性和降解。在这些条件下，在11天的时间内评价ICG的降解。此外，在监测时，在37 ℃孵育温度下在EDTA全血样品中进行ICG的稳定性测量。此外，我们使用质谱（MS）和核磁共振（NMR）分析来鉴定假定的ICG降解化合物。在水溶液和血浆溶液中检测ICG的潜在淬灭效应，浓度范围为0.01-100 g/ml。当在水中稀释并在4 ℃下避光储存时，ICG可稳定储存三天，在此时间跨度内仅损失20%的荧光强度。在37 ℃下在光暴露下在全血中孵育的ICG可稳定5 h。在我们的研究中，我们观察到ICG降解为两种降解化合物，质量分别为m/z 785.32和m/z 1501.57。根据我们的观察，我们建议，如果ICG溶液储存在4 ℃下，则应在制备后1 - 2天内使用ICG。
Fluorescence-based imaging has evolved into an important technology during recent years. Specifically indocyanine green (ICG) has invaded most fields of diagnostic and interventional medicine. Considering the numerous advantages of the substance like the rapid degradation and rare adverse reactions, ICG is currently the most commonly used fluorescing agent. High-performance liquid chromatography (HPLC) was used for measuring absorbance and fluorescence of ICG and its potential degradation compounds. Stability and degradation were evaluated under light exposure or in darkness at various temperatures. Under these conditions, degradation of ICG was evaluated over a period of 11 days. Additional, stability measurements of ICG were performed in EDTA whole blood samples at 37 degrees C incubation temperature while monitoring. Furthermore, we used mass spectrometric (MS) and nuclear magnetic resonance (NMR) analyses for the identification of supposed ICG degradation compound. Potential quenching effect of ICG was examined in aqueous and plasma solutions at concentrations ranging from 0.01-100 g ml(-1). When diluted in water and stored at 4 degrees C in the dark, ICG is stable for three days with only 20% of fluorescence intensity lost in this time-span. ICG incubated at 37 degrees C in whole blood under light exposure is stable for 5 h. In our study we observed the degradation of ICG into two degradation compounds with a mass of m/z 785.32 and m/z 1501.57, respectively. Based on our observations we suggest that ICG should be used within one or two days after preparation, if the ICG solution is stored at 4 degrees C.