Polyphenol-Functionalized Hydrogels Using an Interpenetrating Chitosan Network and Investigation of Their Antioxidant Activity
Polyphenol-Functionalized Hydrogels Using an Interpenetrating Chitosan Network and Investigation of Their Antioxidant Activity
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DOI：
10.1007/s13233-018-6001-8
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发表时间：
2018-01-01
影响因子：
2.4
通讯作者：
Kim, Ho-Joong
中科院分区：
文献类型：
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作者：
Kim, Boram;Kang, Byungman;Kim, Ho-Joong
本文制备了多酚改性水凝胶，并对其抗氧化活性进行了研究。首先合成了基于聚甲基丙烯酸2-羟乙酯（pHEMA）的水凝胶，随后用包括交联壳聚糖和p（HEMA）网络的互穿聚合物网络（IPN）结构官能化。通过酰胺偶联反应，用多酚如没食子酸和多巴胺对所得水凝胶进行进一步改性，得到抗氧化剂水凝胶。使用2，2-二苯基-1-苦肼基和2，2 '-连氮基-双（3-乙基-苯并噻唑啉-6-磺酸）自由基清除试验评价所制备的水凝胶的抗氧化活性。没食子酸改性的水凝胶表现出上级的抗氧化活性相比，其多巴胺功能化的同行，这是相关的苯环中的羟基基团的数量。此外，较长的壳聚糖部分提供了更大量的多酚。因此，含有相同多酚但较长壳聚糖部分的水凝胶表现出更强的抗氧化活性。本工作表明，基于壳聚糖-互穿网络结构的抗氧化水凝胶的开发在生物医学器件和眼科材料中显示出巨大的应用潜力。
In this work, polyphenol-modified hydrogels were prepared and their antioxidant activities were investigated. Poly(2-hydroxyethyl methacrylate) (pHEMA)-based hydrogels were first synthesized and subsequently functionalized with an interpenetrating polymer network (IPN) structure comprising crosslinked chitosans and p(HEMA) networks. The resulting hydrogels were further modified with polyphenols such as gallic acid and dopamine through amide coupling reactions to afford the antioxidant hydrogels. The antioxidant activity of the prepared hydrogels were evaluated using 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) radical scavenging assays. The gallic-acid-modified hydrogels exhibited superior antioxidant activity when compared to their dopamine-functionalized counterparts; this was correlated to the number of hydroxyl groups in the benzene ring. Moreover, longer chitosan moieties afforded larger amounts of polyphenols. Thus, hydrogels containing the same polyphenols but longer chitosan moieties exhibited stronger antioxidant activity. This work demonstrates that the development of antioxidant hydrogels based on chitosan-IPN structures shows great potential for application in biomedical devices and ophthalmic materials.