Inability of a variant strain of Anaplasma phagocytophilum to infect mice
Inability of a variant strain of Anaplasma phagocytophilum to infect mice
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DOI：
10.1086/379725
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发表时间：
2003-12-01
影响因子：
6.4
通讯作者：
Levin, ML
中科院分区：
文献类型：
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作者：
Massung, RF;Priestley, RA;Levin, ML
在罗得岛州的几个地点采集了肩胛骨蜱的若虫。聚合酶链反应和DNA测序用于确定嗜吞噬细胞无原体人因子（AP-ha）和与人类疾病无关的遗传变异（ap -变异1）的存在和流行情况。每个队列中剩余的蜱虫被允许以白足（Peromyscus leucopus）或DBA/2 （Mus musculus）小鼠为食至饱。对吸足的蜱和小鼠血液样本进行AP-ha和ap -变异1的检测。尽管很大比例的感染蜱携带ap变异1，但只有AP-ha从小鼠血液样本中扩增出来。另外的蜱虫被喂食免疫功能低下的SCID小鼠，同样，只有AP-ha能够建立感染，并且只有AP-ha可以通过异种诊断检测到。这些数据表明ap -变异1不能在小鼠中建立感染，我们认为ap -变异1有一个替代的自然宿主，可能是白尾鹿。
Nymphal Ixodes scapularis ticks were collected from several sites in Rhode Island. Polymerase chain reaction and DNA sequencing were used to determine the presence and prevalence of Anaplasma phagocytophilum human agent (AP-ha) and a genetic variant not associated with human disease (AP-variant 1). The remaining ticks from each cohort were allowed to feed to repletion on either white-footed (Peromyscus leucopus) or DBA/2 (Mus musculus) mice. The engorged ticks and murine blood samples were evaluated for the presence of AP-ha and AP-variant 1. Although a high percentage of the infecting ticks harbored AP-variant 1, only AP-ha was amplified from the murine blood samples. Additional ticks were fed on immunocompromised SCID mice, and, again, only AP-ha was capable of establishing an infection, and only AP-ha could be detected by xenodiagnosis. These data suggest that AP-variant 1 cannot establish an infection in mice, and we propose that AP-variant 1 has an alternative natural reservoir, possibly white-tailed deer.