How to use dynamic light scattering to improve the likelihood of growing macromolecular crystals.
How to use dynamic light scattering to improve the likelihood of growing macromolecular crystals.
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DOI：
10.1007/978-1-59745-209-0_6
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Borgstahl, Gloria E O
中科院分区：
文献类型：
--
作者：
Borgstahl, Gloria E O
动态光散射（DLS）已成为最有用的结晶诊断工具之一。使用DLS进行晶体筛选的主要目的是帮助研究者了解溶液中大分子的大小分布、稳定性和聚集状态。它也可以用来理解实验变量如何影响聚合。使用市售仪器，DLS易于执行，并且大多数样品是可回收的。最有用的是，样品的均匀性或单分散性，如DLS测量，可以预测结晶性。
Dynamic light scattering (DLS) has become one of the most useful diagnostic tools for crystallization. The main purpose of using DLS in crystal screening is to help the investigator understand the size distribution, stability, and aggregation state of macromolecules in solution. It can also be used to understand how experimental variables influence aggregation. With commercially available instruments, DLS is easy to perform, and most of the sample is recoverable. Most usefully, the homogeneity or monodispersity of a sample, as measured by DLS, can be predictive of crystallizability.