Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging.
Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging.
复制标题
柠檬酸三钠热解制备超小单层石墨烯量子点用于荧光细胞成像
DOI：
10.2147/ijn.s168570
复制
发表时间：
2018
影响因子：
8
通讯作者：
Chen W
中科院分区：
文献类型：
--
作者：
Hong GL;Zhao HL;Deng HH;Yang HJ;Peng HP;Liu YH;Chen W
研究背景精确控制尺寸的超小石墨烯量子点的制备及其生物学应用具有重要意义。方法采用柠檬酸三钠热裂解超滤的方法制备超小尺寸（1.3±0.5 nm）单层GQD。结果GQDs具有蓝色荧光，其发光峰位置与激发波长无关。量子点的量子产率为3.6%，平均荧光寿命为2.78 ns。结论GQDs稳定性好、毒性低，是一种优良的生物显像剂。超小GQDs不仅可以分布在细胞质中，而且可以渗透到细胞核中。我们确保这项工作将为石墨烯材料在纳米医学中的应用增加一个新的维度。
Background The preparation and biological applications of ultra-small graphene quantum dots (GQDs) with accurate-controlled size are of great significance. Methods Here in, we report a novel procedure involving pyrolysis of trisodium citrate and subsequent ultrafiltration for fabricating monolayer GQDs with ultra-small lateral size (1.3±0.5 nm). Results The GQDs exhibit blue photoluminescence with peak position independent of excitation wavelength. The quantum yield of GQDs is measured to be 3.6%, and the average fluorescence lifetime is 2.78 ns. Conclusion Because of high stability and low toxicity, GQDs are demonstrated to be excellent bioimaging agents. The ultra-small GQDs can not only distribute in the cytoplasm but also penetrate into the nuclei. We ensure that this work will add a new dimension to the application of graphene materials for nanomedicine.