Alien DNA introgression and wheat DNA rearrangements in a stable wheat line derived from the early generation of distant hybridization
Alien DNA introgression and wheat DNA rearrangements in a stable wheat line derived from the early generation of distant hybridization
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DOI：
10.1360/062004-45
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发表时间：
2005-10
期刊：
影响因子：
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通讯作者：
Lianquan Zhang;Dengcai Liu;Zehong Yan;Youliang Zheng
中科院分区：
文献类型：
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作者：
Lianquan Zhang;Dengcai Liu;Zehong Yan;Youliang Zheng
多倍体在植物中很常见。面包或普通小麦（Triticum aestivum L.，2n=42）是由A、B和D三个二倍体基因组组成的异源多倍体的一个很好的例子。近年来，通过模拟普通小麦起源的研究发现，四倍体小麦与节节麦在人工异源六倍体形成的早期就发生了DNA序列和基因表达的变化，这可能有利于新的人工合成六倍体小麦的遗传二倍体化。普通小麦99L2是利用普通小麦与黑麦远缘杂交早代自交选育而成的遗传稳定的新品系。在本研究中，发现至少有两个黑麦DNA片段已渗入99L2。这一结果表明，小麦与外来种之间可能存在一种不同于传统的染色体配对和DNA重组的外源DNA渐渗机制。同时，在外源黑麦DNA片段导入过程中，小麦自身DNA序列也发生了变化。
Polyploidy has been found to be common in plants. Bread or common wheat (Triticum aestivum L., 2n=42) is a good example of allopolyploid made up of three diploid genomes A, B and D. In recent years, by the study of mimicking the origination of common wheat, it was found that changes of DNA sequence and gene expression occurred at the early stages of artificial allohexaploid between tetraploid wheat and Aegilops tauschii, which was probably favorable to genetic diploidization of new synthetic hexaploid wheat. Common wheat 99L2 is a new line stable in genetic, which was derived from the early self-pollinated generation of wide hybrids between common wheat and rye. In this study, it was found that at least two rye DNA segments had been introgressed into 99L2. This result suggested that a mechanism of alien DNA introgression may exist, which was different from the traditional mechanism of chromosome pairing and DNA recombination between wheat and alien species. Meanwhile, during the introgression process of alien rye DNA segments, the changes in DNA sequences of wheat itself occurred.