Dermal organization in scleroderma: The fast Fourier transform and the laser scatter method objectify fibrosis in nonlesional as well as lesional skin
Dermal organization in scleroderma: The fast Fourier transform and the laser scatter method objectify fibrosis in nonlesional as well as lesional skin
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DOI：
10.1038/labinvest.3780136
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发表时间：
2000-08-01
影响因子：
5
通讯作者：
Bos, JD
中科院分区：
文献类型：
--
作者：
de Vries, HJC;Enomoto, DNH;Bos, JD
硬皮病是一种慢性进行性疾病，其特征是真皮纤维化，胶原束平行于表皮。需要简单客观的参数来评估疾病进展和治疗。本文介绍了激光散射法和快速傅立叶变换（FFT）两种测量胶原束方向和间距的方法。采用激光散射法评估病变硬化皮（LS）、非病变硬化皮（nonLS）和对照皮肤（CS）切片的取向比。FFT用于计算胶原束的取向比、变异和间距。参数与局部和平均皮肤评分测量值相关，评分范围为0（正常）至3（严重硬化）。在激光散射法和FFT下，LS的取向比（分别为2.16 +/- 0.33和1.83 +/- 0.62）显著高于CS（分别为1.70 +/- 0.35和1.38 +/- 0.15）。非LS取向比介于LS和CS之间（分别为1.92 +/- 0.15和1.48 +/- 0.44）。与CS（分别为73.8 +/- 15.0和18.9 +/- 1.9 mu m）相比，LS（分别为57.3 +/- 19.4和15.7 +/- 5.6 mu m）显著降低了取向变异和束间距。非LS的取向比（分别为57.2 +/- 29.0和15.6 +/- 6.1 μ m）与LS相似。LS的束比CS更平行，方向变化更小，排列更密。平均皮肤评分与取向比呈线性相关。局部皮肤评分与取向比不呈线性相关。我们的研究结果表明，没有临床硬化的nonLS真皮已经表现出纤维化特征。两种技术均易于使用，适用于硬皮病病变真皮纤维化物化。FFT比激光散射法更准确和可重复性，并允许同时对分析组织切片的位置进行病理评估。未来的研究需要关注临床疾病严重程度与胶原束特征之间的相关性。
Scleroderma, a chronic, progressive disorder, is characterized by dermal fibrosis with collagen bundles orientated parallel to the epidermis. Simple objective parameters to evaluate disease progression and therapies are needed. We describe two methods, the laser scatter method and the fast Fourier transform (FFT), to measure collagen bundle orientation and spacing. Lesional sclerodermic skin (LS), nonlesional sclerodermic skin (nonLS), and control skin (CS) sections were evaluated for orientation ratio using the laser scatter method. The FFT was used to calculate orientation ratio, variation, and spacing of collagen bundles. Parameters were correlated with local and mean skin score measurements, on a scale of 0 (normal) to 3 (severely sclerotic). With both the laser scatter method and the FFT, orientation ratios of LS (respectively, 2.16 +/- 0.33 and 1.83 +/- 0.62) were significantly higher than CS (respectively, 1.70 +/- 0.35 and 1.38 +/- 0.15). NonLS orientation ratios (respectively, 1.92 +/- 0.15 and 1.48 +/- 0.44) were between LS and CS ratios. Orientation variation and bundle spacing of LS (respectively, 57.3 +/- 19.4 and 15.7 +/- 5.6 mu m) were significantly reduced compared to CS (respectively, 73.8 +/- 15.0 and 18.9 +/- 1.9 mu m). NonLS orientation ratios (respectively, 57.2 +/- 29.0 and 15.6 +/- 6.1 mu m) were similar to LS. Bundles in LS are more parallel, show less variation in orientation, and are more densely packed than in CS. There was a linear correlation between mean skin score and orientation ratio. Local skin score was not linearly correlated to orientation ratio. Our findings suggest that nonLS dermis without clinical sclerosis already shows fibrotic characteristics. Both techniques were easy to use and suitable for objectifying dermal fibrosis in scleroderma lesions. FFT is more accurate and reproducible than the laser scatter method and allows simultaneous pathological evaluation of the location of the analyzed tissue sections. Future studies will need to focus on the correlation between clinical disease severity and collagen bundle characteristics.