1,1-dimethylallylcoumarins potently supress both lipopolysaccharide- and interferon-γ-induced nitric oxide generation in mouse macrophage RAW 264.7 cells
1,1-dimethylallylcoumarins potently supress both lipopolysaccharide- and interferon-γ-induced nitric oxide generation in mouse macrophage RAW 264.7 cells
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DOI：
10.1016/s0960-894x(99)00578-8
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发表时间：
2000-01-03
影响因子：
2.7
通讯作者：
Ohigashi, H
中科院分区：
文献类型：
--
作者：
Murakami, A;Gao, GX;Ohigashi, H
我们研究了16个香豆素相关化合物对脂多糖和干扰素γ诱导的小鼠巨噬细胞系RAW 264.7产生一氧化氮的抑制作用。值得注意的是，含有戊烯基团的香豆素(S)被发现具有高度的活性，这一趋势与我们之前的研究一致。在所测试的香豆素中，1，1-二甲基烯丙基香豆素的抑制活性最高。Western blotting分析表明，它们通过抑制诱导型一氧化氮合酶(INOS)蛋白的表达来抑制NO的生成。我们正在进行的研究表明，香豆素是一种重要的天然化合物，可以减少炎症部位过量和延长的NO生成。(C)1999爱思唯尔科学有限公司。保留所有权利。
We investigated the suppressive effects of 16 coumarin-related compounds on both lipopolysaccharide (LPS)- and interferon (IFN)-gamma-induced nitric oxide (NO) generation in a mouse macrophage cell line, RAW 264.7. Notably, coumarins possessing prenyl unit(s) were found to be highly active, a tendency consistent with our previous study. Among the coumarins tested, 1,1-dimethylallylcoumarins showed the highest inhibitory activity. Western blotting analysis revealed that they inhibited NO generation by suppressing inducible NO synthase (iNOS) protein expression. Our ongoing studies suggest that coumarins are prominent natural compounds that attenuate excessive and prolonged NO generation at inflammatory sites. (C) 1999 Elsevier Science Ltd. All rights reserved.