Unsuitability of using the DNPH-coated solid sorbent cartridge for determination of airborne unsaturated carbonyls
Unsuitability of using the DNPH-coated solid sorbent cartridge for determination of airborne unsaturated carbonyls
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DOI：
10.1016/j.atmosenv.2010.09.042
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发表时间：
2011-01-01
影响因子：
5
通讯作者：
Ip, H. S. S.
中科院分区：
文献类型：
--
作者：
Ho, Steven Sai Hang;Ho, K. F.;Ip, H. S. S.
醛和酮的测量通常通过使用涂有2，4-二硝基苯肼（DNPH）的吸附剂柱衍生化来进行。收集的样品用乙腈洗脱，并通过高压液相色谱法与紫外检测器（HPLC/UV）联用进行分析。本文旨在研究人工制品的适用性鉴定不饱和羰基化合物。丙烯醛、巴豆醛、甲基丙烯醛和甲基乙烯基酮（MVK）的动力学测试显示在取样期间形成羰基-DNP-腙，其可进一步与DNPH反应，导致不期望的UV吸收产物[例如，羰基-DNP-腙-DNPH（二聚体）和2（羰基-DNP-腙）-DNPH（三聚体）]。丙烯醛和MVK发生二聚和三聚，而丁烯醛和甲基丙烯醛只发生二聚。聚合产物无疑会影响色谱图的完整性，导致错误鉴定和不准确的定量。在取样和/或样品处理过程中采取的预防措施是否可以避免或最大限度地减少这种人为因素尚未得到深入研究。更常见的情况是，科学家在报告数据时通常会忽视这些人为因素。(C)2010爱思唯尔有限公司保留所有权利。
Measurements of aldehydes and ketones are typically conducted by derivatization using sorbent cartridges coated with 2,4-dinitrophenylhydrazine (DNPH). The collected samples are eluted with acetonitrile and analyzed by high-pressure liquid chromatography coupled with an ultra-violet detector (HPLC/UV). This paper intends to examine artifacts about its suitability in identification of unsaturated carbonyls. Kinetic tests for acrolein, crotonaldehyde, methacrolein and methyl vinyl ketone (MVK) showed formations of carbonyl-DNP-hydrazone during sampling, which could further react with DNPH, resulting in undesired UV absorption products [e.g., carbonyl-DNP-hydrazone-DNPH (dimer) and 2 (carbonyl-DNP-hydrazone)-DNPH (trimer)]. The dimerization and trimerization occurred for acrolein and MVK whereas only dimerization for crotonaldehyde and methacrolein. The polymerization products undoubtedly affect the integrity of the chromatogram, leading to misidentification and inaccurate quantification. Whether precautions taken during sampling and/or sample treatment could avoid or minimize this artifact has not been thoughtfully investigated. More often, such artifacts are usually overlooked by scientists when the data are reported. (C) 2010 Elsevier Ltd. All rights reserved.